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NRF1 antibody

Anti-NRF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33367
Anti-NRF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33367
Anti-NRF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33367
Anti-NRF1 antibody used in Western Blot (WB). GTX33367

Cat No. GTX33367

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 240-449 of human NRF1 (NP_001035199.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nuclear respiratory factor 1 , ALPHA-PAL

Cellular Localization

Nucleus

Background

This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014]

Database

Research Area

DATA IMAGES

Anti-NRF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33367

GTX33367 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX33367 NRF1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-NRF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33367

GTX33367 ICC/IF Image

ICC/IF analysis of PC-12 cells using GTX33367 NRF1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-NRF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33367

GTX33367 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33367 NRF1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-NRF1 antibody used in Western Blot (WB). GTX33367

GTX33367 WB Image

WB analysis of various sample lysates using GTX33367 NRF1 antibody.
Loading : 25ug per lane
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329