GeneTex
  • Country / Location Selection

United States (US)

NRF2 antibody [N2C2], Internal

Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in ChIP assay (ChIP assay). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in Immunoprecipitation (IP). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322

Cat No. GTX103322

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP assay, IHC
Reactivity Human, Mouse, Rat, Zebrafish, Alligator, Plant, Bird
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Observed MW

110 kDa. The observed M.W. is based on the following publication. PMID: 22703241

Positive Control

C8D30 , 3xFlag-human NFE2L2-transfected 293T , MDA-MB-231 nuclear extract , MDA-MB-231 nuclear extract (30uM tBHQ, 4hr) , HepG2 (10 μM MG132 treatment for 15 hr)

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NRF2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

nuclear factor, erythroid 2 like 2 , HEBP1 , IMDDHH , NRF2

Cellular Localization

Cytoplasm , cytosol , Nucleus

Background

NFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995 [PubMed 7868116]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322

GTX103322 WB Image

NRF2 antibody detects NRF2 protein by western blot analysis. Non-transfected (-) and NRF2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody (GTX103322) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NRF2 antibody [N2C2], Internal used in ChIP assay (ChIP assay). GTX103322

GTX103322 ChIP assay Image

ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-NRF2 antibody. The precipitated DNA was detected by PCR with primer set targeting to GCLC gene locus.

Anti-NRF2 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103322

GTX103322 ICC/IF Image

NRF2 antibody [N2C2], Internal detects NRF2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322

GTX103322 WB Image

Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NRF2 antibody [N2C2], Internal used in Immunoprecipitation (IP). GTX103322

GTX103322 IP Image

Immunoprecipitation of NRF2 protein from HepG2 whole cell extracts using 5 μg of NRF2 antibody [N2C2], Internal (GTX103322).
Western blot analysis was performed using NRF2 antibody [N2C2], Internal (GTX103322).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322

GTX103322 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322

GTX103322 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322

GTX103322 WB Image

Untreated (–) and treated (+) MDA-MB-231 nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:1000.

Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322

GTX103322 WB Image

Untreated (–) and treated (+) RAW264.7 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NRF2 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103322

GTX103322 IHC-P Image

NRF2 antibody [N2C2], Internal detects NRF2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NRF2 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103322

GTX103322 ICC/IF Image

NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322

GTX103322 WB Image

Untreated (–) and treated (+) HepG2 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322

GTX103322 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE
REVIEW
There are currently no reviews for NRF2 antibody [N2C2], Internal (GTX103322). Be the first to share your experience with this product.
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169