GeneTex
United States (US)

NS1 (JEV) antibody

GTX125866_40856_20170721_WB_JEV_18121410_221.jpg
GTX125866_40856_IFA_2_18121410_946.jpg
GTX125866_40856_IFA_18121410_785.jpg

Cat No. GTX125866

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Japanese encephalitis virus
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:50000
ICC/IF 1:100-1:2000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

JEV-infected BHK-21
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Japanese encephalitis virus

Immunogen

Full length Japanese encephalitis virus JEV NS1 Recombinant protein

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Secreted. Host endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side

Research Area

DATA IMAGES
GTX125866_40856_20170721_WB_JEV_18121410_221.jpg

GTX125866 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NS1 (JEV) antibody (GTX125866) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX125866_40856_IFA_2_18121410_946.jpg

GTX125866 ICC/IF Image

Non-structural protein NS1 (Japanese encephalitis virus) antibody detects non-structural protein NS1 (Japanese encephalitis virus) protein by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis viruswere fixed in paraformaldehyde.
Green: non-structural protein NS1 (Japanese encephalitis virus) protein stained by Non-structural protein NS1 (Japanese encephalitis virus) antibody (GTX125866) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX125866_40856_IFA_18121410_785.jpg

GTX125866 ICC/IF Image

Non-structural protein NS1 (Japanese encephalitis virus) antibody detects non-structural protein NS1 (Japanese encephalitis virus) protein by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis viruswere fixed in MeOH.
Green: non-structural protein NS1 (Japanese encephalitis virus) protein stained by Non-structural protein NS1 (Japanese encephalitis virus) antibody (GTX125866) diluted at 1:2000.
Blue: Hoechst 33342 staining.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169