GeneTex
United States (US)

NS1 (JEV) antibody

Anti-NS1 (JEV) antibody used in Western Blot (WB). GTX131369
Anti-NS1 (JEV) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131369

Cat No. GTX131369

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Japanese encephalitis virus
See all JEV NS1 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2500
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

JEV infected BHK-21
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.16 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein encompassing a sequence within the center region of Japanese encephalitis virus JEV NS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Research Area

DATA IMAGES
Anti-NS1 (JEV) antibody used in Western Blot (WB). GTX131369

GTX131369 WB Image

non-structural protein NS1 (JEV) antibody detects non-structural protein NS1 (JEV) protein by western blot analysis. Un-infected (-) and infected (+, JEV infection) BHK-21 whole cell extracts (5 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with non-structural protein NS1 (JEV) antibody (GTX131369) at a dilution of 1:5000.
Anti-NS1 (JEV) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131369

GTX131369 ICC/IF Image

NS1 protein (Japanese encephalitis virus) antibody detects NS1 protein (Japanese encephalitis virus) by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis virus (right) were fixed in methanol.
Green: NS1 (Japanese encephalitis virus) protein stained by NS1 protein (Japanese encephalitis virus) antibody (GTX131369) diluted at 1:2500.
Blue: Hoechst 33342 staining.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169