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NS1' (JEV) antibody

Anti-NS1' (JEV) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132499
Anti-NS1' (JEV) antibody used in Western Blot (WB). GTX132499

Cat No. GTX132499

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Japanese encephalitis virus
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:5000
Not tested in other applications.

Positive Control

JEV infected BHK21 cells
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Japanese encephalitis virus NS1'. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-NS1' (JEV) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132499

GTX132499 ICC/IF Image

JEV NS1' antibody detects JEV NS1' protein by immunofluorescent analysis. Sample: BHK-21 cells mock (left) and infected with JEV (right) were fixed in 4% paraformaldehyde. Green: JEV NS1' protein stained by JEV NS1' antibody (GTX132499) diluted at 1:5000. Blue: DAPI staining.

Anti-NS1' (JEV) antibody used in Western Blot (WB). GTX132499

GTX132499 WB Image

Non-infected (–) and infected (+) BHK-21 cell extracts (30 μg) were separated by 10% SDS-PAGE. Left: the membrane was blotted with JEV NS1 antibody (GTX131370) diluted at 1:5000; Right: the membrane was blotted with JEV NS1' antibody (GTX132499) diluted at 1:20000.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf