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Japanese encephalitis virus NS2B antibody

Anti-Japanese encephalitis virus NS2B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125972
Anti-Japanese encephalitis virus NS2B antibody used in Western Blot (WB). GTX125972

Cat. No. GTX125972

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Japanese encephalitis virus
Package
100 μl ($399),
25 μl ($169)
See all Japanese encephalitis virus NS2B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

Japanese encephalitis virus infect BHK-21

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Japanese encephalitis virus

Immunogen

Recombinant protein encompassing a sequence within the center region of NS2B (JEV). (Japanese Encephalitis Virus strain Jaoars982) The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176644

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Japanese encephalitis Virus Non-structural protein 2B , Japanese encephalitis Virus NS2B , JEV Non-structural protein 2B , JEV NS2B

Cellular Localization

Protein C: Virion (Potential) , Host membrane; Single-pass membrane protein , Small envelope protein M: Virion (Potential) , Envelope protein E: Virion (Potential) , Host membrane; Multi-pass membrane protein , Non-structural protein 4A: Host membrane; Si

Research Area

DATA IMAGES

Anti-Japanese encephalitis virus NS2B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125972

GTX125972 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Infected BHK-21 cells , using NS2b (Japanese encephalitis virus)(GTX125972) antibody at 1:2000 dilution.

Anti-Japanese encephalitis virus NS2B antibody used in Western Blot (WB). GTX125972

GTX125972 WB Image

Sample (20 ug of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: Japanese encephalitis virus infect BHK-21
12% SDS PAGE
GTX125972 diluted at 1:5000

REFERENCE

Application Reference

Xiaochun Ma et al. Viruses 2022; 14 (4) Construction of a Recombinant Japanese Encephalitis Virus with a Hemagglutinin-Tagged NS2A: A Model for an Analysis of Biological Characteristics and Functions of NS2A during Viral Infection.
Keisuke Tabata et al. J Virol 2021; 95 (15) : e0223420 Endoplasmic Reticulum-Associated Degradation Controls Virus Protein Homeostasis, Which Is Required for Flavivirus Propagation.
Application : WB
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169