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NS3 (JEV) antibody

Anti-NS3 (JEV) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125868
Anti-NS3 (JEV) antibody used in Western Blot (WB). GTX125868
Anti-NS3 (JEV) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125868

Cat No. GTX125868

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Japanese encephalitis virus
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:2000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

Japanese encephalitis virus infected BHK-21
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Japanese encephalitis virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Japanese encephalitis virus (JEV) NS3 protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note: Remains non-covalently associated to NS3 protease

Research Area

DATA IMAGES
Anti-NS3 (JEV) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125868

GTX125868 ICC/IF Image

NS3 (Japanese encephalitis virus) antibody detects NS3 (Japanese encephalitis virus) protein by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis virus (right) were fixed in paraformaldehyde.
Green: NS3 (Japanese encephalitis virus) protein stained by NS3 (Japanese encephalitis virus) antibody (GTX125868) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-NS3 (JEV) antibody used in Western Blot (WB). GTX125868

GTX125868 WB Image

NS3 (Japanese encephalitis virus) antibody detects NS3 (Japanese encephalitis virus) protein by western blot analysis.
A. 10 µg BHK-21 whole cell extract
B. 10 µg whole cell extract of Japanese encephalitis virus infected BHK-21 cells
C. 10 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells
10% SDS-PAGE
NS3 (Japanese encephalitis virus) antibody (GTX125868) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NS3 (JEV) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125868

GTX125868 ICC/IF Image

NS3 (Japanese encephalitis virus) antibody detects NS3 (Japanese encephalitis virus) protein by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis viruswere fixed in MeOH.
Green: NS3 (Japanese encephalitis virus) protein stained by NS3 (Japanese encephalitis virus) antibody (GTX125868) diluted at 1:2000.
Blue: Hoechst 33342 staining.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169