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NS5 (JEV) antibody

Anti-NS5 (JEV) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131359
Anti-NS5 (JEV) antibody used in Western Blot (WB). GTX131359

Cat No. GTX131359

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Japanese encephalitis virus
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:3000
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Positive Control

JEV infected BHK-21 cells
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Japanese encephalitis virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Japanese encephalitis virus (JEV) NS5 protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

The small proteins NS2A, NS4A and NS4B are hydrophobic, suggesting a possible membrane-related function. Non-structural protein 2B is a required cofactor for the serine protease function of NS3. Serine protease NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS3 serine protease, in association with NS2B, cleaves the polyprotein. RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome, and performs the capping of genomes in the cytoplasm. NS5 methylates viral RNA cap at guanine N-7 and ribose 2'-O positions.

Research Area

DATA IMAGES
Anti-NS5 (JEV) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131359

GTX131359 ICC/IF Image

NS5 protein (Japanese encephalitis virus) antibody detects NS5 protein (Japanese encephalitis virus) by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis virus (right) were fixed in methanol.
Green: NS5 (Japanese encephalitis virus) protein stained by NS5 protein (Japanese encephalitis virus) antibody (GTX131359) diluted at 1:3000.
Blue: Hoechst 33342 staining.

Anti-NS5 (JEV) antibody used in Western Blot (WB). GTX131359

GTX131359 WB Image

NS5 (JEV) antibody detects NS5 (JEV) protein by Western blot analysis. Un-infected (-) and infected (+, JEV infection) BHK-21 whole cell extracts (5 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NS5 (JEV) antibody (GTX131359) at a dilution of 1:3000.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf