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NSD3 antibody [2E9]

Anti-NSD3 antibody [2E9] used in Western Blot (WB). GTX50055

Cat No. GTX50055

Host

Mouse

Clonality

Monoclonal

Clone Name

2E9

Isotype

IgG2b

Application

WB, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1,000 ~ 3,000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

162 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human NSD3 (383-660aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Nuclear Receptor Binding Set Domain Protein 3 , Kmt3F , Kmt3G , Whistle , Whsc1L1 , Pp14328 , Nsd3

Background

This gene encoding NSD3 is related to the Wolf-Hirschhorn syndrome candidate - 1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. NSD3 is Histone methyltransferase which preferentially methylates 'Lys-4' and 'Lys-27' of histone H3 and is amplified in several tumor cell lines and primary breast carcinomas.

Database

Research Area

DATA IMAGES

Anti-NSD3 antibody [2E9] used in Western Blot (WB). GTX50055

GTX50055 WB Image

Cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human NSD3 (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf