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NSD3 antibody [C1C2], Internal

Anti-NSD3 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109396
Anti-NSD3 antibody [C1C2], Internal used in Western Blot (WB). GTX109396
Anti-NSD3 antibody [C1C2], Internal used in Western Blot (WB). GTX109396

Cat No. GTX109396

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

162 kDa. ( Note )

Positive Control

293T , HeLa , Raji , mouse brain

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NSD3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952572

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nuclear receptor binding SET domain protein 3 , KMT3F , KMT3G , WHISTLE , WHSC1L1 , pp14328

Cellular Localization

Nucleus

Background

This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. The function of the protein has not been determined. Two alternatively spliced variants have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NSD3 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109396

GTX109396 ICC/IF Image

NSD3 antibody [C1C2], Internal detects NSD3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NSD3 protein stained by NSD3 antibody [C1C2], Internal (GTX109396) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-NSD3 antibody [C1C2], Internal used in Western Blot (WB). GTX109396

GTX109396 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX109396 diluted at 1:1000

Anti-NSD3 antibody [C1C2], Internal used in Western Blot (WB). GTX109396

GTX109396 WB Image

NSD3 antibody [C1C2], Internal detects NSD3 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 5 % SDS-PAGE, and blotted with NSD3 antibody [C1C2], Internal (GTX109396) diluted by 1:1000

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf