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NSD3 antibody

Anti-NSD3 antibody used in Western Blot (WB). GTX55733
Anti-NSD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55733

Cat No. GTX55733

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

162 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human WHSC1L1 (NP_060248.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nuclear receptor binding SET domain protein 3 , KMT3F , KMT3G , WHISTLE , WHSC1L1 , pp14328

Cellular Localization

Nucleus

Background

This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. Two alternatively spliced variants have been described. [provided by RefSeq, May 2015]

Database

Research Area

DATA IMAGES

Anti-NSD3 antibody used in Western Blot (WB). GTX55733

GTX55733 WB Image

WB analysis of various sample lysates using GTX55733 NSD3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-NSD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55733

GTX55733 ICC/IF Image

ICC/IF analysis of A549 cells using GTX55733 NSD3 antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf