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NSE antibody [5A4]

Cat. No. GTX10169

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5A4

Isotype

 IgG2a

Application

 WB, IHC-P, ELISA, Sandwich ELISA

Reactivity

 Human
Package
100 μg ($399)
See all NSE products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX10169, Detection : GTX28328
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified NSE from human brain.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_366969

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

enolase 2 , HEL-S-279 , NSE

Cellular Localization

Cytoplasm , Cell membrane

Background

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE

Application Reference

Masi I et al. Cell Rep 2021; 34 (9) : 108800 Endothelin-1 drives invadopodia and interaction with mesothelial cells through ILK.
Application : WB
Reactivity : Human
Lee YK et al. Cancers (Basel) 2020; 12 (9) Mitochondrial Respiratory Defect Enhances Hepatoma Cell Invasiveness via STAT3/NFE2L1/STX12 Axis.
Fischer N et al. Int J Cancer 2010; 126 (9) : 2133-42 Detection of Merkel cell polyomavirus (MCPyV) in Merkel cell carcinoma cell lines: cell morphology and growth phenotype do not reflect presence of the virus.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399