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NSE antibody [5D3]

Anti-NSE antibody [5D3] used in Western Blot (WB). GTX60625
Anti-NSE antibody [5D3] used in IHC (Paraffin sections) (IHC-P). GTX60625
Anti-NSE antibody [5D3] used in IHC (Paraffin sections) (IHC-P). GTX60625
Anti-NSE antibody [5D3] used in ELISA (ELISA). GTX60625
Anti-NSE antibody [5D3] used in Flow cytometry (FACS). GTX60625

Cat. No. GTX60625

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5D3

Isotype

 IgG1

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($409)
See all NSE products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human NSE (AA: 251-433) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

enolase 2 , HEL-S-279 , NSE

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-NSE antibody [5D3] used in Western Blot (WB). GTX60625

GTX60625 WB Image

WB analysis of Mouse brain (1), NIH3T3 (2), and C6 (3) cell lysate using GTX60625 NSE antibody [5D3].

Anti-NSE antibody [5D3] used in IHC (Paraffin sections) (IHC-P). GTX60625

GTX60625 IHC-P Image

IHC-P analysis of liver cancer tissue using GTX60625 NSE antibody [5D3].

Anti-NSE antibody [5D3] used in IHC (Paraffin sections) (IHC-P). GTX60625

GTX60625 IHC-P Image

IHC-P analysis of lung cancer tissue using GTX60625 NSE antibody [5D3].

Anti-NSE antibody [5D3] used in ELISA (ELISA). GTX60625

GTX60625 ELISA Image

ELISA analysis of antigen using GTX60625 NSE antibody [5D3].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-NSE antibody [5D3] used in Flow cytometry (FACS). GTX60625

GTX60625 FACS Image

FACS analysis of HeLa cells using GTX60625 NSE antibody [5D3].
Green : NSE
Red : negative control

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409