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NSE antibody [C1C3]

Anti-NSE antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX113428
Anti-NSE antibody [C1C3] used in Western Blot (WB). GTX113428
Anti-NSE antibody [C1C3] used in Western Blot (WB). GTX113428
Anti-NSE antibody [C1C3] used in IHC (Frozen sections) (IHC-Fr). GTX113428
Anti-NSE antibody [C1C3] used in Western Blot (WB). GTX113428
Anti-NSE antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113428
Anti-NSE antibody [C1C3] used in Western Blot (WB). GTX113428
Anti-NSE antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113428

Cat. No. GTX113428

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all NSE products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431

Product Note

This antibody was raised against human NSE (ENO2) protein, but it may cross react other family members based on sequence homology.

Predict Reactivity

Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NSE. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950201

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

enolase 2 , HEL-S-279 , NSE

Cellular Localization

Cytoplasm , Cell membrane

Background

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NSE antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX113428

GTX113428 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using NSE antibody [C1C3] (GTX113428) at 1:300 dilution.

Anti-NSE antibody [C1C3] used in Western Blot (WB). GTX113428

GTX113428 WB Image

Sample (30 μg of whole cell lysate)
A: whole zebrafish
B: zebrafish muscle
10% SDS PAGE
GTX113428 diluted at 1:1000

Anti-NSE antibody [C1C3] used in Western Blot (WB). GTX113428

GTX113428 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
10% SDS PAGE
GTX113428 diluted at 1:1000

Anti-NSE antibody [C1C3] used in IHC (Frozen sections) (IHC-Fr). GTX113428

GTX113428 IHC-Fr Image

NSE antibody [C1C3] detects NSE protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: NSE protein stained by NSE antibody [C1C3] (GTX113428) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NSE antibody [C1C3] used in Western Blot (WB). GTX113428

GTX113428 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX113428 diluted at 1:10000

Anti-NSE antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113428

GTX113428 ICC/IF Image

NSE antibody [C1C3] detects NSE protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NSE protein stained by NSE antibody [C1C3] (GTX113428) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-NSE antibody [C1C3] used in Western Blot (WB). GTX113428

GTX113428 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSE antibody [C1C3] (GTX113428) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NSE antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113428

GTX113428 ICC/IF Image

The data was published in the journal Stem Cells Int in 2019. PMID: 31065279

REFERENCE

Application Reference

Cort?s-Medina LV et al. Stem Cells Int 2019; 2019 : 7627148 Neuronal Transdifferentiation Potential of Human Mesenchymal Stem Cells from Neonatal and Adult Sources by a Small Molecule Cocktail.
Lorena V. Cort?s-Medina et al. Stem Cells International 2019; 2019 : Article ID 7627148 Neuronal Transdifferentiation Potential of Human Mesenchymal Stem Cells from Neonatal and Adult Sources by a Small Molecule Cocktail.
Application : ICC/IF
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169