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NSE antibody [MSVA-451M] HistoMAX™

HistoMAX
Anti-NSE antibody [MSVA-451M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04388
Anti-NSE antibody [MSVA-451M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04388
Anti-NSE antibody [MSVA-451M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04388

Cat. No. GTX04388

Host

Mouse

Clonality

Monoclonal

Clone Name

MSVA-451M

Isotype

IgG1

Application

IHC-P

Reactivity

Human
Package
500 μl ($519),
100 μl ($299)
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on testing result, pH 9.0 showed a strongest staining pattern. However, pH 9.0 results in only a slight reduction of the staining intensity as compared to pH 9.0. Therefore, the pH 7.8 was an optimal for manual staining because of the better tissue preservation at pH 7.8 than at pH 9.0.

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

In the colon, axons and ganglion cells in lamina propria and muscular wall must show at least a moderate NSE staining, while epithelial and lymphatic cells remain negative.

Negative Control

In the colon, epithelial and lymphatic cells do not show NSE staining.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA (Please contact us for PBS only format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide of human NSE gamma (around aa416-433)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

enolase 2 , HEL-S-279 , NSE

Cellular Localization

Cytoplasm , Cell membrane

Background

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-NSE antibody [MSVA-451M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04388

GTX04388 IHC-P Image

IHC-P analysis of human cerebellum tissue section using GTX04388 NSE antibody [MSVA-451M] HistoMAX™.
A strong NSE immunostaining is seen in the granule cell layer and in the fibres of the white matter of the cerebellum.

Anti-NSE antibody [MSVA-451M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04388

GTX04388 IHC-P Image

IHC-P analysis of human adrenal pheochromocytoma tissue section using GTX04388 NSE antibody [MSVA-451M] HistoMAX™.
Strong NSE immunostaining in an adrenal pheochromocytoma.

Anti-NSE antibody [MSVA-451M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04388

GTX04388 IHC-P Image

IHC-P analysis of human langerhans islets tissue section using GTX04388 NSE antibody [MSVA-451M] HistoMAX™.
Positive NSE immunostaining of islets of Langerhans.

REFERENCE

There are currently no references for NSE antibody [MSVA-451M] HistoMAX™ (GTX04388). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for NSE antibody [MSVA-451M] HistoMAX™ (GTX04388). Be the first to share your experience with this product.
Package List Price ($)
$ 519
$ 299