PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer. Based on testing result, pH 9.0 showed a strongest staining pattern. However, pH 7.8 results in only a slight reduction of the staining intensity as compared to pH 9.0. Therefore, the pH 7.8 was an optimal for manual staining because of the better tissue preservation at pH 7.8 than at pH 9.0.
|
Not tested in other applications.
Calculated MW
Positive Control
In the colon, axons and ganglion cells in lamina propria and muscular wall must show at least a moderate NSE staining, while epithelial and lymphatic cells remain negative.
Negative Control
In the colon, epithelial and lymphatic cells do not show NSE staining.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide of human NSE gamma (around aa416-433)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
enolase 2 , HEL-S-279 , NSE
Cellular Localization
Cytoplasm , Cell membrane
Background
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-NSE antibody [MSVA-451M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04388](/templates/default/js/lightbox/images/loading.gif "Anti-NSE antibody [MSVA-451M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04388")
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GTX04388 IHC-P Image
IHC-P analysis of human cerebellum tissue using GTX04388 NSE antibody [MSVA-451M] HistoMAX™.
A strong NSE immunostaining is seen in the granule cell layer and in the fibres of the white matter of the cerebellum.
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GTX04388 IHC-P Image
IHC-P analysis of human adrenal pheochromocytoma tissue using GTX04388 NSE antibody [MSVA-451M] HistoMAX™.
Strong NSE immunostaining in an adrenal pheochromocytoma.
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GTX04388 IHC-P Image
IHC-P analysis of human islets of Langerhans from pancreas tissue using GTX04388 NSE antibody [MSVA-451M] HistoMAX™.
Positive NSE immunostaining of islets of Langerhans.
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REFERENCE
There are currently no references for NSE antibody [MSVA-451M] HistoMAX™ (GTX04388). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for NSE antibody [MSVA-451M] HistoMAX™ (GTX04388). Be the first to share your experience with this product.
![Anti-NSE antibody [MSVA-451M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04388](/upload/website/prouct_img/small/GTX04388/GTX04388_20230728_IHC-P_216_23072723_804.webp "Anti-NSE antibody [MSVA-451M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04388")
![Anti-NSE antibody [MSVA-451M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04388](/upload/website/prouct_img/small/GTX04388/GTX04388_20230728_IHC-P_346_23072723_533.webp "Anti-NSE antibody [MSVA-451M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04388")
![Anti-NSE antibody [MSVA-451M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04388](/upload/website/prouct_img/small/GTX04388/GTX04388_20230728_IHC-P_86_23072722_150.webp "Anti-NSE antibody [MSVA-451M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04388")
![Anti-NSE antibody [MSVA-451M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04388](/upload/website/prouct_img/normal/GTX04388/GTX04388_20230728_IHC-P_216_23072723_804.webp "Anti-NSE antibody [MSVA-451M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04388")
![Anti-NSE antibody [MSVA-451M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04388](/upload/website/prouct_img/normal/GTX04388/GTX04388_20230728_IHC-P_346_23072723_533.webp "Anti-NSE antibody [MSVA-451M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04388")
![Anti-NSE antibody [MSVA-451M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04388](/upload/website/prouct_img/normal/GTX04388/GTX04388_20230728_IHC-P_86_23072722_150.webp "Anti-NSE antibody [MSVA-451M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04388")