APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
mouse brain , rat brain
Predict Reactivity
Human, Zebrafish, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.54 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse NSE. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886889
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
enolase 2, gamma neuronal , AI837106 , D6Ertd375e , Eno-2 , NSE
Cellular Localization
Cytoplasm , Cell membrane
Background
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
Database
Research Area
DATA IMAGES
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GTX133249 IHC-P Image
NSE antibody detects NSE protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NSE antibody (GTX133249) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX133249 ICC/IF Image
NSE antibody detects NSE protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NSE protein stained by NSE antibody (GTX133249) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX133249 IHC-Fr Image
NSE antibody detects NSE protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: NSE protein stained by NSE antibody (GTX133249) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX133249 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSE antibody (GTX133249) diluted at 1:5000.
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GTX133249 IHC-P Image
NSE antibody detects NSE protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NSE antibody (GTX133249) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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REVIEW
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