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NSE antibody

Anti-NSE antibody used in IHC (Paraffin sections) (IHC-P). GTX133249
Anti-NSE antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133249
Anti-NSE antibody used in IHC (Frozen sections) (IHC-Fr). GTX133249
Anti-NSE antibody used in Western Blot (WB). GTX133249
Anti-NSE antibody used in IHC (Paraffin sections) (IHC-P). GTX133249

Cat No. GTX133249

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all NSE products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse NSE. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

enolase 2, gamma neuronal , AI837106 , D6Ertd375e , Eno-2 , NSE

Cellular Localization

Cytoplasm , Cell membrane

Background

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

Database

Research Area

DATA IMAGES

Anti-NSE antibody used in IHC (Paraffin sections) (IHC-P). GTX133249

GTX133249 IHC-P Image

NSE antibody detects NSE protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NSE antibody (GTX133249) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NSE antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133249

GTX133249 ICC/IF Image

NSE antibody detects NSE protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NSE protein stained by NSE antibody (GTX133249) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NSE antibody used in IHC (Frozen sections) (IHC-Fr). GTX133249

GTX133249 IHC-Fr Image

NSE antibody detects NSE protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: NSE protein stained by NSE antibody (GTX133249) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NSE antibody used in Western Blot (WB). GTX133249

GTX133249 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSE antibody (GTX133249) diluted at 1:5000.

Anti-NSE antibody used in IHC (Paraffin sections) (IHC-P). GTX133249

GTX133249 IHC-P Image

NSE antibody detects NSE protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NSE antibody (GTX133249) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169