GeneTex
United States (US)

NSE antibody

GTX133249_42606_20161219_IHC-P_R_18121410_534.jpg
GTX133249_42606_20170824_IFA_18121410_605.jpg
GTX133249_42606_20161012_IHC-Fr_R_18121410_363.jpg
GTX133249_42606_20160922_WB_M_R_18121410_508.jpg
GTX133249_42606_20161219_IHC-P_M_18121410_965.jpg

Cat No. GTX133249

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Mouse, Rat
See all NSE products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse NSE. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AI837106 antibody , D6Ertd375e antibody , Eno2 antibody , NSE antibody , NSE antibody , enolase 2, gamma neuronal antibody

Cellular Localization

Cytoplasm (By similarity) , Cell membrane (By similarity)

Background

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

Database

Research Area

DATA IMAGES
GTX133249_42606_20161219_IHC-P_R_18121410_534.jpg

GTX133249 IHC-P Image

NSE antibody detects NSE protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NSE antibody (GTX133249) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX133249_42606_20170824_IFA_18121410_605.jpg

GTX133249 ICC/IF Image

NSE antibody detects NSE protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NSE protein stained by NSE antibody (GTX133249) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
GTX133249_42606_20161012_IHC-Fr_R_18121410_363.jpg

GTX133249 IHC-Fr Image

NSE antibody detects NSE protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: NSE protein stained by NSE antibody (GTX133249) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
GTX133249_42606_20160922_WB_M_R_18121410_508.jpg

GTX133249 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSE antibody (GTX133249) diluted at 1:5000.
GTX133249_42606_20161219_IHC-P_M_18121410_965.jpg

GTX133249 IHC-P Image

NSE antibody detects NSE protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NSE antibody (GTX133249) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169