GeneTex

  • Country / Location Selection

United States (US)

NSE antibody

Anti-NSE antibody used in Western Blot (WB). GTX85462
Anti-NSE antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85462

Cat. No. GTX85462

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ICC/IF, IHC

Reactivity

 Human, Mouse, Rat
Package
50 μg ($399)
See all NSE products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:10000-1:20000
ICC/IF Assay dependent
IHC 1:10000-1:20000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the NSE-2 gene product, but are shared between the human (NP_001966, NCBI) and rat (AAA41119, NCBI) sequences.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

enolase 2 , HEL-S-279 , NSE

Cellular Localization

Cytoplasm , Cell membrane

Background

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-NSE antibody used in Western Blot (WB). GTX85462

GTX85462 WB Image

WB analysis of NSE protein using GTX85462 NSE antibody.

Anti-NSE antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85462

GTX85462 ICC/IF Image

ICC/IF analysis of neonatal mouse brains using GTX85462 NSE antibody.
Green : Primary antibody
Red : GFAP
Blue : DAPI

REFERENCE

There are currently no references for NSE antibody (GTX85462). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for NSE antibody (GTX85462). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399