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NSF antibody [9G7-3]

Cat No. GTX12368

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 9G7-3

Isotype

 IgG2b

Application

 WB, IP

Reactivity

 Human, Mouse, Rat, Rabbit, Sheep, Bovine, Dog, Hamster, Pig, Monkey
Package
50 μg ($329)
See all NSF products

APPLICATION

Application Note

IP: Use at an assay dependent dilution. WB: Use at a concentration of 0.25 μg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 82.6 kDa). Optimal dilutions/concentrations should be determined by the end user.

Positive Control

Rat Brain Tissue Extract.

PROPERTIES

Form

Liquid

Buffer

Preservative: NoneConstituents: 50% Glycerol, PBS, 0.1mM PMSF

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Recombinant full length protein (Cow).

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Cytoplasmic

Background

N ethylmaleimide sensitive factor (NSF) is an ~83 kDa protein (predicted MW) which was initially isolated based on its ability to restore intercisternal Golgi transport to N ethylmaleimide treated membranes. NSF was subsequently discovered to be involved in a variety of membrane fusion events. NSF is an ATPase with two ATP binding domains that are essential for membrane fusion and homo oligomerization. In order to interact with target membranes, NSF requires another set of soluble proteins called Soluble NSF attachment proteins (SNAPs). NSF has been shown to play a prominent role in synaptic vesicle fusion. Together with synaptotagmin and a SNAP, NSF modulates the interaction of SNAP25, VAMP, and syntaxin, in an ATP dependent manner, to form a complex with a sedimentation coefficient of 20 S. This complex may represent an intermediate involved in synaptic vesicle docking and fusion.

Research Area

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 329