GeneTex
United States (US)

NSF antibody [NSF-1]

NSF-antibody-NSF-1_WB_GTX16681-1_18121411_618.jpg

Cat No. GTX16681

Host Mouse
Clonality Monoclonal
Clone Name NSF-1
Isotype IgG
Application WB, ICC/IF, IP, IHC
Reactivity Human, Mouse, Rat, Bovine
APPLICATION

Application Note

IF: Use at an assay dependent dilution. IHC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/2000. Detects a band of approximately 76 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

83 kDa. ( Note )

Positive Control

Rat PC12
PROPERTIES

Form

Liquid

Buffer

Preservative: None Constituents: 50% Glycerol, 20mM Sodium phosphate, 150mM Sodium chloride. pH 7.5

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full length protein (Human).

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

N-Ethylmaleimide Sensitive Factor, Vesicle Fusing Atpase,Sec18,Skd2,Nsf

Cellular Localization

Cytoplasmic

Background

N ethylmaleimide sensitive factor (NSF) is an ~83 kDa protein (predicted MW) which was initially isolated based on its ability to restore intercisternal Golgi transport to N ethylmaleimide treated membranes. NSF was subsequently discovered to be involved in a variety of membrane fusion events. NSF is an ATPase with two ATP binding domains that are essential for membrane fusion and homo oligomerization. In order to interact with target membranes, NSF requires another set of soluble proteins called Soluble NSF attachment proteins (SNAPs). NSF has been shown to play a prominent role in synaptic vesicle fusion. Together with synaptotagmin and a SNAP, NSF modulates the interaction of SNAP25, VAMP, and syntaxin, in an ATP dependent manner, to form a complex with a sedimentation coefficient of 20 S. This complex may represent an intermediate involved in synaptic vesicle docking and fusion.

Database

Research Area

DATA IMAGES
NSF-antibody-NSF-1_WB_GTX16681-1_18121411_618.jpg

GTX16681 WB Image

Western blot (10% SDS-PAGE) of 3T3 (lane 1), PC12 (lane 2), MDBK (lane 3) and normal human fibroblasts (lane 4) probed with clone NSF-1 anti-NSF at 0.5 µg/ml. The diffuse bands at ~70 kDa were also present on a blot incubated with second antibody alone (data not shown).

Package List Price ($)
$ 319