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NSF antibody

Anti-NSF antibody used in Western Blot (WB). GTX110275
Anti-NSF antibody used in Western Blot (WB). GTX110275
Anti-NSF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110275
Anti-NSF antibody used in Western Blot (WB). GTX110275
Anti-NSF antibody used in IHC (Paraffin sections) (IHC-P). GTX110275

Cat. No. GTX110275

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NSF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

293T , mouse brain , rat brain

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NSF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951026

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

N-ethylmaleimide sensitive factor, vesicle fusing ATPase , SEC18 , SKD2

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-NSF antibody used in Western Blot (WB). GTX110275

GTX110275 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX110275 diluted at 1:1000

Anti-NSF antibody used in Western Blot (WB). GTX110275

GTX110275 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NSF antibody (GTX110275) diluted at 1:1000.

Anti-NSF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110275

GTX110275 ICC/IF Image

NSF antibody detects NSF protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NSF protein stained by NSF antibody (GTX110275) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NSF antibody used in Western Blot (WB). GTX110275

GTX110275 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NSF antibody (GTX110275) diluted at 1:1000.

Anti-NSF antibody used in IHC (Paraffin sections) (IHC-P). GTX110275

GTX110275 IHC-P Image

NSF antibody detects NSF protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
NSF antibody (GTX110275) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169