GeneTex
United States (US)

NSFL1C antibody [N1C2]

GTX105035_39953_20150402_IHC_M_19011506_327.jpg
GTX105035_39953_20150710_IP_19011506_969.jpg
GTX105035_39953_WB_M_brain_19011506_439.jpg
GTX105035_IHC_19011506_252.jpg
GTX105035_WB_19011506_646.jpg

Cat No. GTX105035

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NSFL1C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC3347 antibody, UBX1 antibody, UBXD10 antibody, UBXN2C antibody, dJ776F14.1 antibody, p47 antibody, NSFL1C antibody, NSFL1 cofactor p47 antibody, SHP1 homolog antibody, UBX domain-containing protein 2C antibody, p97 cofactor p47 antibody, NSFL1 (p97) cofactor (p47) antibody

Cellular Localization

Nucleus (By similarity) , Golgi apparatus , Golgi stack (By similarity)

Background

N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Database

DATA IMAGES
GTX105035_39953_20150402_IHC_M_19011506_327.jpg

GTX105035 IHC-P Image

NSFL1C antibody [N1C2] detects NSFL1C protein at cytoplasm and nucleus on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NSFL1C antibody [N1C2] (GTX105035) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105035_39953_20150710_IP_19011506_969.jpg

GTX105035 IP Image

Immunoprecipitation of NSFL1C protein from A431 whole cell extracts using 5 μg of NSFL1C antibody [N1C2] (GTX105035).
Western blot analysis was performed using NSFL1C antibody [N1C2] (GTX105035).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX105035_39953_WB_M_brain_19011506_439.jpg

GTX105035 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX105035 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105035_IHC_19011506_252.jpg

GTX105035 IHC-P Image

Immunohistochemical analysis of paraffin-embedded differential N/T pair Lung SCC cancer xenograft, using NSFL1C(GTX105035) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105035_WB_19011506_646.jpg

GTX105035 WB Image

Sample(30 µg whole cell lysate)
A:293T
B:A431(GTX27909)
C:H1299
10% SDS PAGE
GTX105035 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169