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NSFL1C antibody [N1C2]

Anti-NSFL1C antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX105035
Anti-NSFL1C antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX105035
Anti-NSFL1C antibody [N1C2] used in Western Blot (WB). GTX105035
Anti-NSFL1C antibody [N1C2] used in Immunoprecipitation (IP). GTX105035
Anti-NSFL1C antibody [N1C2] used in Western Blot (WB). GTX105035

Cat. No. GTX105035

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all NSFL1C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431 , H1299

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NSFL1C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951027

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NSFL1 cofactor , P47 , UBX1 , UBXD10 , UBXN2C , dJ776F14.1

Cellular Localization

Nucleus , Golgi apparatus , Golgi stack

Background

N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NSFL1C antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX105035

GTX105035 IHC-P Image

Immunohistochemical analysis of paraffin-embedded differential N/T pair Lung SCC cancer xenograft, using NSFL1C(GTX105035) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NSFL1C antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX105035

GTX105035 IHC-P Image

NSFL1C antibody [N1C2] detects NSFL1C protein at cytoplasm and nucleus on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NSFL1C antibody [N1C2] (GTX105035) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NSFL1C antibody [N1C2] used in Western Blot (WB). GTX105035

GTX105035 WB Image

Sample(30 μg whole cell lysate)
A:293T
B:A431(GTX27909)
C:H1299
10% SDS PAGE
GTX105035 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NSFL1C antibody [N1C2] used in Immunoprecipitation (IP). GTX105035

GTX105035 IP Image

Immunoprecipitation of NSFL1C protein from A431 whole cell extracts using 5 μg of NSFL1C antibody [N1C2] (GTX105035).
Western blot analysis was performed using NSFL1C antibody [N1C2] (GTX105035).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-NSFL1C antibody [N1C2] used in Western Blot (WB). GTX105035

GTX105035 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX105035 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169