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NSFL1C antibody

Anti-NSFL1C antibody used in Western Blot (WB). GTX33368
Anti-NSFL1C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33368
Anti-NSFL1C antibody used in Immunoprecipitation (IP). GTX33368

Cat. No. GTX33368

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all NSFL1C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human NSFL1C (NP_057227.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NSFL1 cofactor , P47 , UBX1 , UBXD10 , UBXN2C , dJ776F14.1

Cellular Localization

Nucleus

Background

N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8. [provided by RefSeq, May 2011]

Database

Research Area

DATA IMAGES

Anti-NSFL1C antibody used in Western Blot (WB). GTX33368

GTX33368 WB Image

WB analysis of various sample lysates using GTX33368 NSFL1C antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-NSFL1C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33368

GTX33368 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33368 NSFL1C antibody.
Blue : DAPI

Anti-NSFL1C antibody used in Immunoprecipitation (IP). GTX33368

GTX33368 IP Image

IP analysis of A549 cell lysate using GTX33368 NSFL1C antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399