GeneTex
  • Country / Location Selection

United States (US)

NSFL1C antibody

Anti-NSFL1C antibody used in Immunoprecipitation (IP). GTX33368
Anti-NSFL1C antibody used in Western Blot (WB). GTX33368
Anti-NSFL1C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33368

Cat No. GTX33368

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50-1:100
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human NSFL1C (NP_057227.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NSFL1 cofactor , P47 , UBX1 , UBXD10 , UBXN2C , dJ776F14.1

Cellular Localization

Nucleus

Background

N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8. [provided by RefSeq, May 2011]

Database

Research Area

DATA IMAGES

Anti-NSFL1C antibody used in Immunoprecipitation (IP). GTX33368

GTX33368 IP Image

IP analysis of A431 cells using GTX33368 NSFL1C antibody.
IP reaction : 200μg extracts / 3μg antibody antibody
Dilution : 1:1000

Anti-NSFL1C antibody used in Western Blot (WB). GTX33368

GTX33368 WB Image

WB analysis of various sample lysates using GTX33368 NSFL1C antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-NSFL1C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33368

GTX33368 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33368 NSFL1C antibody.
Blue : DAPI

REFERENCE

There are currently no references for NSFL1C antibody (GTX33368). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for NSFL1C antibody (GTX33368). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf