Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with NSUN4 antibody [N1C2] (GTX120442) diluted at 1:500.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NSUN4(GTX120442) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with NSUN4 antibody [N1C2] (GTX120442) diluted at 1:500.

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
GTX120442 diluted at 1:1000

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSUN4 antibody [N1C2] (GTX120442) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.