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NSUN6 antibody [N3C3]

Anti-NSUN6 antibody [N3C3] used in Western Blot (WB). GTX107157
Anti-NSUN6 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107157

Cat. No. GTX107157

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NSUN6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NSUN6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951031

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NOP2/Sun RNA methyltransferase 6 , 4933414E04Rik , ARL5B-AS1 , NOPD1

Background

May have S-adenosyl-L-methionine-dependent methyl-transferase activity.

Database

DATA IMAGES

Anti-NSUN6 antibody [N3C3] used in Western Blot (WB). GTX107157

GTX107157 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSUN6 antibody [N3C3] (GTX107157) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NSUN6 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107157

GTX107157 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NSUN6(GTX107157) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169