GeneTex
United States (US)

NT5C2 antibody [N2C1], Internal

GTX105719_IHC_19011506_599.jpg
GTX105719_WB_19011506_929.jpg
GTX105719_39946_WB_M_liver_19011506_885.jpg

Cat No. GTX105719

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse liver

Predict Reactivity

Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NT5C2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GMP antibody, NT5B antibody, PNT5 antibody, cN-II antibody, NT5C2 antibody, cytosolic 5'-nucleotidase II antibody, "5'-nucleotidase (purine), cytosolic type B antibody", IMP-specific 5'-NT antibody, purine 5' nucleotidase antibody, cytosolic purine 5'-nucleotidase antibody, "5'-nucleotidase, cytosolic II antibody"

Cellular Localization

Cytoplasm

Background

Purine 5-prime-nucleotidase (EC 3.1.3.5) preferentially hydrolyzes inosine 5-prime-monophosphate (IMP) and other purine nucleotides, and is allosterically activated by various compounds, including ATP. The enzyme is exclusively located in the cytoplasmic matrix of cells and may have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX105719_IHC_19011506_599.jpg

GTX105719 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using NT5C2(GTX105719) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105719_WB_19011506_929.jpg

GTX105719 WB Image

Sample(30 ug whole cell lysate)
A:H1299
B:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX105719 diluted at 1:1000

GTX105719_39946_WB_M_liver_19011506_885.jpg

GTX105719 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX105719 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169