GeneTex
United States (US)

NT5C2 antibody

GTX115657_40310_WB_18121410_944.jpg
GTX115657_40310_IHC_18121410_464.jpg

Cat No. GTX115657

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

HepG2 , HCT116

Predict Reactivity

Mouse, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human NT5C2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

5'-Nucleotidase, Cytosolic Ii,Gmp,Nt5B,Pnt5,Spg45,Spg65,Cn-Ii,Nt5C2

Cellular Localization

Cytoplasm

Background

Purine 5-prime-nucleotidase (EC 3.1.3.5) preferentially hydrolyzes inosine 5-prime-monophosphate (IMP) and other purine nucleotides, and is allosterically activated by various compounds, including ATP. The enzyme is exclusively located in the cytoplasmic matrix of cells and may have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX115657_40310_WB_18121410_944.jpg

GTX115657 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
7.5% SDS PAGE
GTX115657 diluted at 1:1000

GTX115657_40310_IHC_18121410_464.jpg

GTX115657 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using NT5C2(GTX115657) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169