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NTCP antibody

Anti-NTCP antibody used in Western Blot (WB). GTX17430
Anti-NTCP antibody used in IHC (Paraffin sections) (IHC-P). GTX17430

Cat. No. GTX17430

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μg ($449)
See all NTCP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
IHC-P 0.5-1μg/ml
Not tested in other applications.

Calculated MW

38.12 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1% Na₂HPO₄, 0.45% NaCl, 2.5% BSA

Preservative

0.025% Thimerosal, 0.025% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

500 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human SLC10A1(141-154aa YSRGIYDGDLKDKV), different from the related rat and mouse sequences by one amino acid.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887562

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Membrane

Background

The protein encoded by this gene belongs to the sodium/bile acid cotransporter family, which are integral membrane glycoproteins that participate in the enterohepatic circulation of bile acids. Two homologous transporters are involved in the reabsorption of bile acids; the ileal sodium/bile acid cotransporter with an apical cell localization that absorbs bile acids from the intestinal lumen, bile duct and kidney, and the liver-specific sodium/bile acid cotransporter, represented by this protein, that is found in the basolateral membranes of hepatocytes. Bile acids are the catabolic product of cholesterol metabolism, hence this protein is important for cholesterol homeostasis. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-NTCP antibody used in Western Blot (WB). GTX17430

GTX17430 WB Image

WB analysis of various samples using GTX17430 NTCP antibody.
Lane 1 : rat liver tissue lysate at 50ug
Lane 2 : mouse liver tissue lysate at 50ug
Dilution : 0.5 μg/mL

Anti-NTCP antibody used in IHC (Paraffin sections) (IHC-P). GTX17430

GTX17430 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX17430 NTCP antibody.

REFERENCE

Application Reference

Balasubramaniyan N et al. Hepatol Commun 2019; 3 (12) : 1674-1686 Up-regulation of miR-let7a-5p Leads to Decreased Expression of ABCC2 in Obstructive Cholestasis.
Natarajan Balasubramaniyan et al. hepatology Communications 2019; (Epub) Up?regulation of miR?let7a?5p Leads to Decreased Expression of ABCC2 in Obstructive Cholestasis.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Sodium Azide.pdf
Thimerosal.pdf
Package List Price ($)
$ 449