GeneTex
United States (US)

NTE antibody

GTX115501_41339_IHC_R_18121410_287.jpg
GTX115501_41339_IHC_M_18121410_758.jpg
GTX115501_41339_20141128_WB_18121410_820.jpg
GTX115501_41339_20141128_WB_R_brain_18121410_122.jpg

Cat No. GTX115501

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

150 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR-32 , SK-N-AS , rat brain

Predict Reactivity

Human, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human NTE. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Patatin Like Phospholipase Domain Containing 6,Bnhs,Lnms,Nte,Ntemnd,Omcs,Spg39,Ipla2Delta,Sws,Pnpla6

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type I membrane protein; Cytoplasmic side

Background

This gene encodes a phospholipase that deacetylates intracellular phosphatidylcholine to produce glycerophosphocholine. It is thought to function in neurite outgrowth and process elongation during neuronal differentiation. The protein is anchored to the cytoplasmic face of the endoplasmic reticulum in both neurons and non-neuronal cells. Mutations in this gene result in autosomal recessive spastic paraplegia, and the protein is the target for neurodegeneration induced by organophosphorus compounds and chemical warfare agents. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115501_41339_IHC_R_18121410_287.jpg

GTX115501 IHC-P Image

NTE antibody detects NTE protein at cytosol on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
NTE antibody (GTX115501) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115501_41339_IHC_M_18121410_758.jpg

GTX115501 IHC-P Image

NTE antibody detects NTE protein at cytosol on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
NTE antibody (GTX115501) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115501_41339_20141128_WB_18121410_820.jpg

GTX115501 WB Image

NTE antibody detects NTE protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NTE antibody (GTX115501) diluted by 1:1000.

GTX115501_41339_20141128_WB_R_brain_18121410_122.jpg

GTX115501 WB Image

NTE antibody detects NTE protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 5 % SDS-PAGE, and the membrane was blotted with NTE antibody (GTX115501) diluted by 1:1000.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf