APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.2-2.5 ug/ml |
2-10 ug/ml |
Not tested in other applications.
Calculated MW
Positive Control
COLO205 , HEK293 , MDA-MB-231 , MDCK , NMuMG
PROPERTIES
Form
Liquid
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to an Internal region of Human NUCB2
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nucleobindin 2 , HEL-S-109 , NEFA
Cellular Localization
Golgi apparatus,Membrane,Cytoplasm,Secreted,Endoplasmic reticulum
Background
This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011]
Database
Research Area
DATA IMAGES
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GTX47487 WB Image
WB analysis of HEK293, MDCK, NMuMG, and MDA-MB-231 cells using GTX47487 NUCB2 antibody at 1:1000. Loading amount:50 ug.
Lane 1 : HEK293
Lane 2 : MDCK
Lane 3 : NMuMG
Lane 4 : MDA-MB-231
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GTX47487 IHC-P Image
IHC-P analysis of human intestine tissue using GTX47487 NUCB2 antibody at 4.0-8.0μg/ml.
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GTX47487 WB Image
WB analysis of COLO205 cells using GTX47487 NUCB2 antibody at 1μg/ml.
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REFERENCE
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REVIEW
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