ICC/IF analysis of P19 cells using GTX00739 NUCB2 antibody. Nucleobindin 2 was localized to the cytoplasm near the nucleus in undifferentiated P19 cells, and its immunoreactivity in the cytoplasm was increased when P19 cells were induced to differentiate into neurons.
Left panel: undifferentiated murine embryonal carcinoma P19 cells (UD)
Right panels: enriched post-mitotic neurons (PN)

WB analysis of various mouse tissue samples using GTX00739 NUCB2 antibody.
Dilution : 1:1000

IHC-Fr analysis of cerebral cortex (parietal lobe) of mouse brain tissue section using GTX00739 NUCB2 antibody.
At higher magnification (B, C), fine granular immunoreactive materials are observed at both neuronal dendrites (arrows) and perikarya (asterisks) in the layer IV (arrowhead B in A) and subplate (arrowhead C in A) of the cerebral cortex.
Scale bars : 100 μm (A) and 10μm (B and C).

WB analysis of mouse cerebral cortex using GTX00739 NUCB2 antibody.
The apparent molecular mass (55~57 kDa) is larger than the calculated one (50 kDa) and the band is broad, which may reflect post-translational modifications.
Dilution : 1:1000
Loading : 10 μg