Immunoprecipitation of NUCB2 protein from 293T whole cell extracts using 5 μg of NUCB2 antibody (GTX130943).
Western blot analysis was performed using NUCB2 antibody (GTX130943) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

NUCB2 antibody detects NUCB2 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NUCB2 antibody (GTX130943) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

NUCB2 antibody detects NUCB2 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NUCB2 protein stained by NUCB2 antibody (GTX130943) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

NUCB2 antibody detects NUCB2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NUCB2 antibody (GTX130943) diluted at 1:1000.