APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
NIH-3T3 , BCL-1 , 293T , A431 , HeLa , HepG2
Predict Reactivity
Rat, Bovine, Pig, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human NUDC. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11179797
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nuclear distribution C, dynein complex regulator , HNUDC , MNUDC , NPD011
Cellular Localization
Cytoplasm , cytoskeleton , Nucleus
Background
NudC was first identified as a regulator of nuclear movement in the asexual reproductive cycle of the filamentous fungus Aspergillus nidulans. Human NUDC is a nuclear movement protein that associates with dynein (see DYNC1H1; MIM 600112) (Aumais et al., 2003 [PubMed 12679384]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX117622 ICC/IF Image
NUDC antibody detects NUDC protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NUDC protein stained by NUDC antibody (GTX117622) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX117622 WB Image
Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
10% SDS PAGE
GTX117622 diluted at 1:1000
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GTX117622 WB Image
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: BCL-1
10% SDS PAGE
GTX117622 diluted at 1:1000
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REFERENCE
There are currently no references for NUDC antibody (GTX117622). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for NUDC antibody (GTX117622). Be the first to share your experience with this product.
