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NUDT21 antibody

Anti-NUDT21 antibody used in IHC (Paraffin sections) (IHC-P). GTX115535
Anti-NUDT21 antibody used in Western Blot (WB). GTX115535
Anti-NUDT21 antibody used in IHC (Paraffin sections) (IHC-P). GTX115535
Anti-NUDT21 antibody used in IHC (Paraffin sections) (IHC-P). GTX115535
Anti-NUDT21 antibody used in Immunoprecipitation (IP). GTX115535

Cat. No. GTX115535

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat, Entamoeba histolytica
Package
100 μl ($399),
25 μl ($169)
See all NUDT21 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NUDT21. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885377

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nudix hydrolase 21 , CFIM25 , CPSF5

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. This gene encodes the 25kD subunit of the protein complex, which is composed of four polypeptides. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NUDT21 antibody used in IHC (Paraffin sections) (IHC-P). GTX115535

GTX115535 IHC-P Image

NUDT21 antibody detects NUDT21 protein at nucleus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
NUDT21 antibody (GTX115535) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NUDT21 antibody used in Western Blot (WB). GTX115535

GTX115535 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NUDT21 antibody (GTX115535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NUDT21 antibody used in IHC (Paraffin sections) (IHC-P). GTX115535

GTX115535 IHC-P Image

NUDT21 antibody detects NUDT21 protein at nucleus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
NUDT21 antibody (GTX115535) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NUDT21 antibody used in IHC (Paraffin sections) (IHC-P). GTX115535

GTX115535 IHC-P Image

NUDT21 antibody detects NUDT21 protein at nucleus on rat middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat middle brain.
NUDT21 antibody (GTX115535) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NUDT21 antibody used in Immunoprecipitation (IP). GTX115535

GTX115535 IP Image

Immunoprecipitation of NUDT21 protein from SK-N-AS whole cell extracts using 5 μg of NUDT21 antibody (GTX115535).
Western blot analysis was performed using NUDT21 antibody (GTX115535).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

Application Reference

Avila-Bonilla RG et al. Biosci Rep 2023; 43 (2) Comparative genomics and interactomics of polyadenylation factors for the prediction of new parasite targets: Entamoeba histolytica as a working model.
Application : WB, IP
Reactivity : Entamoeba histolytica
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169