GeneTex
United States (US)

NUDT21 antibody

GTX115535_41262_IHC_M_2_18121410_933.jpg
GTX115535_41262_IHC_R_18121410_418.jpg
GTX115535_41262_20150430_IP_18121410_168.jpg
GTX115535_41262_WB_18121410_449.jpg
GTX115535_41262_IHC_M_18121410_184.jpg

Cat No. GTX115535

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

0.97mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NUDT21. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CFIM25 Antibody, CPSF5 Antibody, nudix (nucleoside diphosphate linked moiety X)-type motif 21 Antibody

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. This gene encodes the 25kD subunit of the protein complex, which is composed of four polypeptides. [provided by RefSeq]
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Database

DATA IMAGES
GTX115535_41262_IHC_M_2_18121410_933.jpg

GTX115535 IHC-P Image

NUDT21 antibody detects NUDT21 protein at nucleus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
NUDT21 antibody (GTX115535) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115535_41262_IHC_R_18121410_418.jpg

GTX115535 IHC-P Image

NUDT21 antibody detects NUDT21 protein at nucleus on rat middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat middle brain.
NUDT21 antibody (GTX115535) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115535_41262_20150430_IP_18121410_168.jpg

GTX115535 IP Image

Immunoprecipitation of NUDT21 protein from SK-N-AS whole cell extracts using 5 μg of NUDT21 antibody (GTX115535).
Western blot analysis was performed using NUDT21 antibody (GTX115535).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX115535_41262_WB_18121410_449.jpg

GTX115535 WB Image

NUDT21 antibody detects NUDT21 protein by Western blot analysis.
A. 30 µg SK-N-SH whole cell lysate/extract
B. 30 µg IMR32 whole cell lysate/extract
C. 30 µg SK-N-AS whole cell lysate/extract
12 % SDS-PAGE
NUDT21 antibody (GTX115535) dilution: 1:1000

GTX115535_41262_IHC_M_18121410_184.jpg

GTX115535 IHC-P Image

NUDT21 antibody detects NUDT21 protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
NUDT21 antibody (GTX115535) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169