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NUF2 antibody [GT312]

Anti-NUF2 antibody [GT312] used in Immunoprecipitation (IP). GTX632247
Anti-NUF2 antibody [GT312] used in Western Blot (WB). GTX632247

Cat No. GTX632247

Host Mouse
Clonality Monoclonal
Clone Name GT312
Isotype IgG1
Application WB, IP
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

HeLa , HeLa NE
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ndc80 Kinetochore Complex Component Nuf2,Cdca1,Ct106,Nuf2R,Nuf2

Cellular Localization

Nucleus , Kinetochore

Background

This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-NUF2 antibody [GT312] used in Immunoprecipitation (IP). GTX632247

GTX632247 IP Image

Immunoprecipitation of NUF2 protein from HeLa whole cell extracts using 5 μg of NUF2 antibody [GT312] (GTX632247) .
Western blot analysis was performed using NUF2 antibody [GT312] (GTX632247).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-NUF2 antibody [GT312] used in Western Blot (WB). GTX632247

GTX632247 WB Image

HeLa whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NUF2 antibody [GT312] (GTX632247) diluted at 1:1000.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169