NUF2 antibody [GT312]

Cat. No. GTX632247

Host	Mouse
Clonality	Monoclonal
Clone Name	GT312
Isotype	IgG1
Application	WB, IHC-P, IP
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all NUF2 products

Datasheet
Document

View product citations for antibody GTX632247 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	Assay dependent
IP	1:100-1:500

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

HeLa

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein corresponding to human NUF2

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888300

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NUF2 component of NDC80 kinetochore complex , CDCA1 , CT106 , NUF2R

Cellular Localization

Nucleus , Kinetochore

Background

This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq]

Database

Gene ID: 83540 NUF2
UniProt: Q9BZD4 NUF2

Research Area

DATA IMAGES

GTX632247 IHC-P Image

NUF2 antibody [GT312] detects NUF2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
NUF2 stained by NUF2 antibody [GT312] (GTX632247) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632247 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NUF2 antibody [GT312] (GTX632247) diluted at 1:1000.

GTX632247 IP Image

Immunoprecipitation of NUF2 protein from HeLa whole cell extracts using 5 μg of NUF2 antibody [GT312] (GTX632247) .
Western blot analysis was performed using NUF2 antibody [GT312] (GTX632247) diluted at 1:500.
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

REFERENCE

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SDS
PBS.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations