HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NUF2 antibody [GT644] (GTX632184) diluted at 1:1000.

Immunoprecipitation of NUF2 protein from HeLa whole cell extracts using 5 μg of NUF2 antibody [GT644] (GTX632184) .
Western blot analysis was performed using NUF2 antibody [GT644] (GTX632184) diluted at 1:500.
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.