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NUF2 antibody

Anti-NUF2 antibody used in Western Blot (WB). GTX00733
Anti-NUF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00733

Cat No. GTX00733

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Chicken
Package
100 μl ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:5000
ICC/IF 1:200-1:1000

Note :

ICC/IF
Recommend using methanol as the fixation buffer.

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum with 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide CGGDSYAKIDEKTAELKRKMFKMS corresponding to the C-terminus region of human Nuf2

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NUF2 component of NDC80 kinetochore complex , CDCA1 , CT106 , NUF2R

Cellular Localization

Nucleus

Background

This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-NUF2 antibody used in Western Blot (WB). GTX00733

GTX00733 WB Image

WB analysis of HeLa whole cell lysate using GTX00733 NUF2 antibody.
Dilution : 1:5000

Anti-NUF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00733

GTX00733 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00733 NUF2 antibody. Nuf2 protein (green) actively localizes to kinetochores at the stages of prometaphase to anaphase.
Green : Primary antibody
Violet : DAPI
Dilution : 1:500
Fixation : Methanol

REFERENCE

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REVIEW

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Package List Price ($)
$ 359