Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NULP1 antibody [C1C3] (GTX102102) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NULP1(GTX102102) antibody at 1:200 dilution.

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX102102 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using NULP1(GTX102102) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min