Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NUMB antibody [N1N3] (GTX111715) diluted at 1:1000.

Rat tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NUMB antibody [N1N3] (GTX111715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX111715 diluted at 1:1000