GeneTex
United States (US)

NUMB antibody

GTX30689_1490_WB_18121410_149.jpg
GTX30689_248_ICC-IF_18121410_250.jpg
GTX30689_1489_WB_18121410_329.jpg

Cat No. GTX30689

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat, Chicken
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2 - 0.5 μg/ml
ICC/IF 1:500
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Specificity/Sensitivity

This is specific for all four isoforms of the NUMB protein.
PROPERTIES

Form

Liquid

Buffer

Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3.50mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

A synthetic peptide made to a C-terminal region of mouse NUMB (between residues 600-653). [UniProt# Q9QZS3]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Numb Endocytic Adaptor Protein,Nb,Numb

Cellular Localization

Membrane, Peripheral membrane protein

Background

Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage.

Database

Research Area

DATA IMAGES
GTX30689_1490_WB_18121410_149.jpg

GTX30689 WB Image

WB analysis of A431 cell lysate using GTX30689 NUMB antibody.

GTX30689_248_ICC-IF_18121410_250.jpg

GTX30689 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX30689 NUMB antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:500

GTX30689_1489_WB_18121410_329.jpg

GTX30689 WB Image

WB analysis of A431 cell lysate using GTX30689 NUMB antibody.
Dilution : 0.5 μg/ml
Loading : 20μg

Package List Price ($)
$ 319