APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.2-1μg/ml |
| 2.5-3.8μg/ml |
|
Note :
IHC-P
Human Uterus shows textured nuclear staining of endometrial cells.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89862-PEP).
Product Note
This antibody is expected to recognise both reported isoforms (NP_009103.2; NP_705931.1).
Predict Reactivity
Mouse, Rat, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-ELHKILLEKKDA, from the C Terminus of the protein sequence according to NP_009103.2; NP_705931.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nucleoporin 50 , NPAP60 , NPAP60L
Background
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX89862 WB Image
WB analysis of Jurkat lysate using GTX89862 NUP50 antibody, C-term.
Dilution : 0.5μg/ml
Loading : 30μg protein in RIPA buffer
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GTX89862 IHC-P Image
IHC-P analysis of human uterus using GTX89862 NUP50 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml
|
REFERENCE
There are currently no references for NUP50 antibody, C-term (GTX89862). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for NUP50 antibody, C-term (GTX89862). Be the first to share your experience with this product.
