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NUP50 antibody [C3], C-term

Anti-NUP50 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106428
Anti-NUP50 antibody [C3], C-term used in Western Blot (WB). GTX106428
Anti-NUP50 antibody [C3], C-term used in Western Blot (WB). GTX106428
Anti-NUP50 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106428

Cat. No. GTX106428

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all NUP50 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

mouse testis

Predict Reactivity

Bovine, Chicken, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NUP50. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037557

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nucleoporin 50 , NPAP60 , NPAP60L

Cellular Localization

Nucleus , nuclear pore complex , Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side

Background

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NUP50 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106428

GTX106428 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human normal colon, using NUP50(GTX106428) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NUP50 antibody [C3], C-term used in Western Blot (WB). GTX106428

GTX106428 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NUP50 antibody [C3], C-term (GTX106428) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 17625072

Anti-NUP50 antibody [C3], C-term used in Western Blot (WB). GTX106428

GTX106428 WB Image

NUP50 antibody [C3], C-term detects NUP50 protein by Western blot analysis.
A. 50 μg mouse testis lysate/extract
10 % SDS-PAGE
NUP50 antibody [C3], C-term (GTX106428) dilution: 1:1000

Anti-NUP50 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106428

GTX106428 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using NUP50(GTX106428) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169