GeneTex
United States (US)

NUP50 antibody [C3], C-term

GTX106428_39890_IHC_19011506_686.jpg
GTX106428_39890_IFA_19011506_764.jpg
GTX106428_WB_19011506_641.jpg
GTX106428_39890_20170825_WB_19011506_609.jpg
GTX106428_39890_WB_M_testis_19011506_273.jpg

Cat No. GTX106428

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

H1299 , Jurkat , Raji , NCI-H929 , mouse testis

Predict Reactivity

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NUP50. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC39961 antibody, NPAP60 antibody, NPAP60L antibody, NUP50 antibody, nucleoporin Nup50 antibody, nuclear pore-associated protein 60L antibody, nuclear pore complex protein Nup50 antibody, 50 kDa nucleoporin antibody, nucleoporin 50kDa antibody

Cellular Localization

Nucleus , nuclear pore complex , Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side (By similarity)

Background

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

DATA IMAGES
GTX106428_39890_IHC_19011506_686.jpg

GTX106428 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human normal colon, using NUP50(GTX106428) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106428_39890_IFA_19011506_764.jpg

GTX106428 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using NUP50(GTX106428) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

GTX106428_WB_19011506_641.jpg

GTX106428 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX106428 diluted at 1:1000

GTX106428_39890_20170825_WB_19011506_609.jpg

GTX106428 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NUP50 antibody [C3], C-term (GTX106428) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106428_39890_WB_M_testis_19011506_273.jpg

GTX106428 WB Image

NUP50 antibody [C3], C-term detects NUP50 protein by Western blot analysis.
A. 50 µg mouse testis lysate/extract
10 % SDS-PAGE
NUP50 antibody [C3], C-term (GTX106428) dilution: 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169