Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
Assay dependent |
Assay dependent |
Not tested in other applications.
Product Note
For ICC/IF application, it works on S. cerevisiae, S. pombe, and T. etrahymena but is not suitable for human. Recommend GTX00695 NUP98 antibody [21A10] on human samples.
Form
Liquid
Buffer
Filter-sterilized PBS, 50% glycerol
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Tetrahymena
Immunogen
Synthetic peptides containing conserved N-terminal sequence, GLFG, of Nup98 protein of Tetrahymena thermophila:
Peptide 1; 1-MFGNTGGGGLFGNTQTQQTGGGLFGQPQQ-29
Peptide 2; 646-SNPTQGGGLFGAANPGLGG-664
Epitope determined: FGxxN
Purification
Purified IgG
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.