NUP98 antibody [21A10]

ICC/IF analysis of S. cereviciae cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10].
Green : Primary antibody
Violet : DAPI
Dilution :
13C2 or 21A10 : 1:10
2H10 : 10 μg/ml

ICC/IF analysis of HeLa cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. The signal at the nuclear periphery with 21A10 mAb was much higher and the background lower than that of 2H10 and 13C2 antibodies.
Green : Primary antibody
Violet : DAPI
Dilution : 0.5 μg/ml
Fixation : Cold Methanol (-30 degree C) for 30 min

WB analysis of S. pombe cell l extracts using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. Left and right lanes represent specimens from a wild type strain and an S. pombe strain in which Nup98 was chromosomally replaced with Nup98-GFP, respectively.
Dilution :
13C2 or 21A10 : 1:10
2H10 : 1 μg/ml

ICC/IF analysis of Tetrahymena themophila cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. 13C2 mAb was highly specific to the macronucleus. In contrast, in addition to clear macronuclear staining, 21A10 mAb also stained the micronuclear periphery. This indicates that 21A10 mAb recognizes Nups localizing to the micronucleus such as Nup308 in addition to MacNup98A. Neither mABs 2H10 nor 414 could stain nuclear periphery of Tetrahymena.
Green : Primary antibody
Violet : DAPI
Dilution : 0.5 μg/ml
Fixation : Cold Methanol (-30 degree C) for 30 min

ICC/IF analysis of S. pombe cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10].
Green : Primary antibody
Violet : DAPI
Fixation : 4% PFA for 10 min, treated with 0.6 mg/ml Zymolyase 100T at 3 degree C for 70 min
Permeabilization : 1% Triton X-100 for 1 min

Summary of the suitability of GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10] for immunological applications.

WB analysis of HeLa whole cell lysate using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10].
Dilution : 0.4 μg/ml

WB analysis of Tetrahymena themophila cells or Tetrahymena themophila cells overexpressing GFP-MacNup98A using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10].
Diamonds and asterisks represent uncharacterized proteins.

WB analysis of S. cereviciae celll extracts using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10].
Dilution :
13C2 or 21A10 : 1:10
2H10 : 20 μg/ml