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NUP98 antibody [21A10]

Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695
Anti-NUP98 antibody [21A10] used in  (). GTX00695
Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695
Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695
Anti-NUP98 antibody [21A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00695
Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695
Anti-NUP98 antibody [21A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00695
Anti-NUP98 antibody [21A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00695
Anti-NUP98 antibody [21A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00695

Cat No. GTX00695

Host

Mouse

Clonality

Monoclonal

Clone Name

21A10

Isotype

IgG1

Application

WB, ICC/IF

Reactivity

Human, Mouse, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Tetrahymena
Package
100 μg ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

198 kDa. ( Note )

Specificity/Sensitivity

For WB application, it works on S. cerevisiae and S. pombe nut may be not suitbale for human, T. etrahymena and yeasts. Recommend GTX00693 NUP98 antibody [13C2] on human, T. etrahymena and yeasts samples.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Tetrahymena

Immunogen

Synthetic peptides containing conserved N-terminal sequence, GLFG, of Nup98 protein of Tetrahymena thermophila.
Peptide 1; 1-MFGNTGGGGLFGNTQTQQTGGGLFGQPQQ-29
Peptide 2; 646-SNPTQGGGLFGAANPGLGG-664
Epitope determined: GLF

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nucleoporin 98 , ADIR2 , NUP196 , NUP96

Cellular Localization

nucleoplasm

Background

Nuclear pore complexes (NPCs) regulate the transport of macromolecules between the nucleus and cytoplasm, and are composed of many polypeptide subunits, many of which belong to the nucleoporin family. This gene belongs to the nucleoporin gene family and encodes a 186 kDa precursor protein that undergoes autoproteolytic cleavage to generate a 98 kDa nucleoporin and 96 kDa nucleoporin. The 98 kDa nucleoporin contains a Gly-Leu-Phe-Gly (GLGF) repeat domain and participates in many cellular processes, including nuclear import, nuclear export, mitotic progression, and regulation of gene expression. The 96 kDa nucleoporin is a scaffold component of the NPC. Proteolytic cleavage is important for targeting of the proteins to the NPC. Translocations between this gene and many other partner genes have been observed in different leukemias. Rearrangements typically result in chimeras with the N-terminal GLGF domain of this gene to the C-terminus of the partner gene. Alternative splicing results in multiple transcript variants encoding different isoforms, at least two of which are proteolytically processed. Some variants lack the region that encodes the 96 kDa nucleoporin. [provided by RefSeq, Feb 2016]

Database

DATA IMAGES

Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695

GTX00695 WB Image

WB analysis of S. cereviciae celll extracts using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10].
Dilution :
13C2 or 21A10 : 1:10
2H10 : 20 μg/ml

Anti-NUP98 antibody [21A10] used in  (). GTX00695

GTX00695 Image

Summary of the suitability of GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10] for immunological applications.

Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695

GTX00695 WB Image

WB analysis of Tetrahymena themophila cells or Tetrahymena themophila cells overexpressing GFP-MacNup98A using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10].
Diamonds and asterisks represent uncharacterized proteins.

Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695

GTX00695 WB Image

WB analysis of S. pombe cell l extracts using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. Left and right lanes represent specimens from a wild type strain and an S. pombe strain in which Nup98 was chromosomally replaced with Nup98-GFP, respectively.
Dilution :
13C2 or 21A10 : 1:10
2H10 : 1 μg/ml

Anti-NUP98 antibody [21A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00695

GTX00695 ICC/IF Image

ICC/IF analysis of S. pombe cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10].
Green : Primary antibody
Violet : DAPI
Fixation : 4% PFA for 10 min, treated with 0.6 mg/ml Zymolyase 100T at 3 degree C for 70 min
Permeabilization : 1% Triton X-100 for 1 min

Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695

GTX00695 WB Image

WB analysis of HeLa whole cell lysate using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10].
Dilution : 0.4 μg/ml

Anti-NUP98 antibody [21A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00695

GTX00695 ICC/IF Image

ICC/IF analysis of Tetrahymena themophila cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. 13C2 mAb was highly specific to the macronucleus. In contrast, in addition to clear macronuclear staining, 21A10 mAb also stained the micronuclear periphery. This indicates that 21A10 mAb recognizes Nups localizing to the micronucleus such as Nup308 in addition to MacNup98A. Neither mABs 2H10 nor 414 could stain nuclear periphery of Tetrahymena.
Green : Primary antibody
Violet : DAPI
Dilution : 0.5 μg/ml
Fixation : Cold Methanol (-30 degree C) for 30 min

Anti-NUP98 antibody [21A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00695

GTX00695 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. The signal at the nuclear periphery with 21A10 mAb was much higher and the background lower than that of 2H10 and 13C2 antibodies.
Green : Primary antibody
Violet : DAPI
Dilution : 0.5 μg/ml
Fixation : Cold Methanol (-30 degree C) for 30 min

Anti-NUP98 antibody [21A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00695

GTX00695 ICC/IF Image

ICC/IF analysis of S. cereviciae cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10].
Green : Primary antibody
Violet : DAPI
Dilution :
13C2 or 21A10 : 1:10
2H10 : 10 μg/ml

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 359