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NUP98 antibody [N3C1], Internal

Anti-NUP98 antibody [N3C1], Internal used in Immunoprecipitation (IP). GTX114157
Anti-NUP98 antibody [N3C1], Internal used in Western Blot (WB). GTX114157

Cat No. GTX114157

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

198 kDa. ( Note )

Positive Control

Jurkat , Raji

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human NUP98. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619089

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nucleoporin 98 , ADIR2 , NUP196 , NUP96

Cellular Localization

Nucleus , nuclear pore complex , Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side

Background

Signal-mediated nuclear import and export proceed through the nuclear pore complex (NPC), which is comprised of approximately 50 unique proteins collectively known as nucleoporins. The 98 kD nucleoporin is generated through a biogenesis pathway that involves synthesis and proteolytic cleavage of a 186 kD precursor protein. This cleavage results in the 98 kD nucleoporin as well as a 96 kD nucleoporin, both of which are localized to the nucleoplasmic side of the NPC. Rat studies show that the 98 kD nucleoporin functions as one of several docking site nucleoporins of transport substrates. The human gene has been shown to fuse to several genes following chromsome translocatons in acute myelogenous leukemia (AML) and T-cell acute lymphocytic leukemia (T-ALL). This gene is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described. [provided by RefSeq]

Database

DATA IMAGES

Anti-NUP98 antibody [N3C1], Internal used in Immunoprecipitation (IP). GTX114157

GTX114157 IP Image

Immunoprecipitation of NUP98 protein from HeLa whole cell extracts using 5 μg of NUP98 antibody [N3C1], Internal (GTX114157).
Western blot analysis was performed using NUP98 antibody [N3C1], Internal (GTX114157) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-NUP98 antibody [N3C1], Internal used in Western Blot (WB). GTX114157

GTX114157 WB Image

Sample (30 ug of whole cell lysate)
A: JurKat
7.5% SDS PAGE
GTX114157 diluted at 1:1000

REFERENCE

There are currently no references for NUP98 antibody [N3C1], Internal (GTX114157). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for NUP98 antibody [N3C1], Internal (GTX114157). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169