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NUR77 antibody

Anti-NUR77 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100797
Anti-NUR77 antibody used in Western Blot (WB). GTX100797
Anti-NUR77 antibody used in Western Blot (WB). GTX100797
Anti-NUR77 antibody used in Western Blot (WB). GTX100797

Cat No. GTX100797

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

HepG2 , HepG2 (treatment 1% Hypoxia for 24 hr) , NUR77 tansfected 293T cell , mouse liver

Predict Reactivity

Rat, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human NUR77. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

nuclear receptor subfamily 4 group A member 1 , GFRP1 , HMR , N10 , NAK-1 , NGFIB , NP10 , NUR77 , TR3

Cellular Localization

Nucleus

Background

This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-NUR77 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100797

GTX100797 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NUR77(GTX100797) antibody at 1:500 dilution.

Anti-NUR77 antibody used in Western Blot (WB). GTX100797

GTX100797 WB Image

Sample (50 µg of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX100797 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NUR77 antibody used in Western Blot (WB). GTX100797

GTX100797 WB Image

Untreated (–) and treated (+) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NUR77 antibody (GTX100797) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NUR77 antibody used in Western Blot (WB). GTX100797

GTX100797 WB Image

Western Blot analysis of NR4A1 expression in transfected 293T cell line by NR4A1 polyclonal antibody.
A: Non-transfected lysate.
B: NR4A1 transfected lysate.
12% SDS PAGE
GTX100797 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169