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NUR77 antibody

Orthogonal
Anti-NUR77 antibody used in Western Blot (WB). GTX100797
Anti-NUR77 antibody used in Western Blot (WB). GTX100797
Anti-NUR77 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100797
Anti-NUR77 antibody used in Western Blot (WB). GTX100797
Anti-NUR77 antibody used in Western Blot (WB). GTX100797

Cat. No. GTX100797

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all NUR77 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

Mouse liver , NR4A1 transfected 293T cell , HepG2 (treatment 1% Hypoxia for 24 hr)

Predict Reactivity

Rat, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human NUR77. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241106

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear receptor subfamily 4 group A member 1 , GFRP1 , HMR , N10 , NAK-1 , NGFIB , NP10 , NUR77 , TR3

Cellular Localization

Nucleus

Background

This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NUR77 antibody used in Western Blot (WB). GTX100797

GTX100797 WB Image

Orthogonal

Untreated and treated Jurkat whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NUR77 antibody (GTX100797) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NUR77 antibody used in Western Blot (WB). GTX100797

GTX100797 WB Image

Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NUR77 antibody (GTX100797) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NUR77 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100797

GTX100797 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NUR77(GTX100797) antibody at 1:500 dilution.

Anti-NUR77 antibody used in Western Blot (WB). GTX100797

GTX100797 WB Image

Sample (50 μg of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX100797 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NUR77 antibody used in Western Blot (WB). GTX100797

GTX100797 WB Image

Western Blot analysis of NR4A1 expression in transfected 293T cell line by NR4A1 polyclonal antibody.
A: Non-transfected lysate.
B: NR4A1 transfected lysate.
12% SDS PAGE
GTX100797 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

NUR77 antibody Cat. No. GTX100797

Rating
( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-NUR77 antibody used in IHC (Paraffin sections) (IHC-P). GTX100797
Date : Anonymous submitted on 10-May-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Kidney tissue
Fixation : Paraffin-embedded
Permeabilization /Antigen retrieval : Citrate buffer, pH 6.0, 0.5Hr
Primary Antibody : 1:500, 4°C, 16Hr
Figure Note : Mouse kidney, 16 week.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169