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Nanog ELISA Pair

Nanog ELISA Pair. GTX500017

Cat. No. GTX500017

Application

 ELISA, Sandwich ELISA

Reactivity

 Human
Package
1 pair ($399)
See all Nanog ELISA Pair products

APPLICATION

Application Note

We recommend using the capture antibody at a concentration of 5μg/mL and the detection antibody at a concentration of 5μg/mL. Optimal dilutions should be determined experimentally by the researcher.

PROPERTIES

Form

Liquid

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Nanog Homeobox , Nanog

Background

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

Research Area

DATA IMAGES

Nanog ELISA Pair. GTX500017

GTX500017 ELISA Image

ELISA detection of NANOG using GTX100863 for capture at a concentration of 5 μg/mL and GTX89079 for detection at a concentration of 5 μg/mL. The HRP-conjugated anti-goat IgG antibody (GTX26741) was diluted at 1:20,000-1:200,000 and used to detect the primary antibody.

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Package List Price ($)
$ 399