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Nanog antibody [1E6C4]

Anti-Nanog antibody [1E6C4] used in Western Blot (WB). GTX83107
Anti-Nanog antibody [1E6C4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83107

Cat. No. GTX83107

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1E6C4

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Nanog products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of NANOG (aa20-166) expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Nanog homeobox

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-Nanog antibody [1E6C4] used in Western Blot (WB). GTX83107

GTX83107 WB Image

WB analysis of NTERA-2 cell lysate using GTX83107 Nanog antibody [1E6C4].

Anti-Nanog antibody [1E6C4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83107

GTX83107 ICC/IF Image

ICC/IF analysis of NTERA-2 cells (left) and HeLa cells (right) using GTX83107 Nanog antibody [1E6C4].
Green : Nanog
Red: Actin filaments

REFERENCE

Clone Reference

Ning B et al. Nat Commun 2017; 8 (1):349 USP26 functions as a negative regulator of cellular reprogramming by stabilising PRC1 complex components
Application : ICC/IF
Reactivity : Mouse
Soltanian S et al. Acta Biochim Biophys Sin (Shanghai) 2014; 46 (8):647-58 Expression analysis of BORIS during pluripotent, differentiated, cancerous, and non-cancerous cell states
Application : ICC/IF
Reactivity : Mouse
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399