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Nanog antibody [5A10]

Anti-Nanog antibody [5A10] used in IHC (Paraffin sections) (IHC-P). GTX57553
Anti-Nanog antibody [5A10] used in Flow cytometry (FACS). GTX57553
Anti-Nanog antibody [5A10] used in Western Blot (WB). GTX57553

Cat. No. GTX57553

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5A10

Isotype

 IgG2a

Application

 WB, IHC-P, FACS

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Nanog products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
FACS Assay dependent
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone 5A10 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Nanog protein.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Nanog homeobox , NANOG , Nanog

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-Nanog antibody [5A10] used in IHC (Paraffin sections) (IHC-P). GTX57553

GTX57553 IHC-P Image

IHC-P analysis of human seminoma tissue using GTX57553 Nanog antibody.
Antigen retrieval: 0.1M sodium citrate buffer
Dilution: 1:50

Anti-Nanog antibody [5A10] used in Flow cytometry (FACS). GTX57553

GTX57553 FACS Image

FACS analysis of Hep3B cells using GTX57553 Nanog antibody.
Cell Number: 1 x 10⁶ cells
Upper panel : negative control, Lower panel: Primary antibody
Antibody amount: 2-5 μg

Anti-Nanog antibody [5A10] used in Western Blot (WB). GTX57553

GTX57553 WB Image

WB analysis of NIH3T3 whole cell lysate using GTX57553 Nanog antibody.
Loading : 35 μg
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399