Nanog antibody [GT3312]

Name: Nanog antibody [GT3312]
Brand: GeneTex
SKU: GTX627421
Price: 419.00 USD
Rating: 4.3 (3 reviews)

Cat. No. GTX627421

3 27

Cat. No. GTX627421

GTX627421 WB Image

NANOG antibody [GT3312] validation by siRNA knock-down.
Upperpanel: NANOG antibody [GT3312] GTX627421
Lower panel: GAPDH antibody (GTX100118)
A. 30 μg Tera-2 whole cell lysate/extract
B. 30 μg whole cell lysate/extract of NANOG siRNA#1-transfected Tera-2 cells
C. 30 μg whole cell lysate/extract of NANOG siRNA#2-transfected Tera-2 cells
D. 30 μg whole cell lysate/extract of NANOG siRNA#3-transfected Tera-2 cells
10% SDS-PAGE
NANOG antibody [GT3312] (GTX627421) dilution: 1:1000
GAPDH antibody (GTX100118) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

1 / 8

GTX627421 WB Image

Sample (20 μg of whole cell lysate)
A: mouse ESC
10% SDS PAGE
GTX627421 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

2 / 8

GTX627421 FCM Image

NANOG antibody [GT3312] detects NANOG protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: NANOG antibody [GT3312] dilution: 1:50

3 / 8

GTX627421 WB Image

NANOG antibody [GT3312] detects NANOG protein by western blot analysis.
A. 30 μg human ESC whole cell lysate/extract
10% SDS-PAGE
NANOG antibody [GT3312] (GTX627421) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

4 / 8

GTX627421 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Nanog antibody [GT3312] (GTX627421) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

5 / 8

GTX627421 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
10% SDS PAGE
GTX627421 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

6 / 8

GTX627421 WB Image

The data was published in the journal Mol Cancer in 2013. PMID: 24094005

7 / 8

GTX627421 WB Image

The data was published in the journal Cancers (Basel) in 2019. PMID: 31878324

8 / 8

This Nanog antibody [GT3312] (GTX627421) is a mouse monoclonal antibody validated for detection of Nanog by WB and FCM. Reactivity has been shown in Human and Mouse. This product is cited in 27 published studies. Knockout/knockdown validation supports antibody specificity.

Host

Mouse
Clonality

Monoclonal
Clone Name

GT3312
Isotype

IgG2b
Applications

WB FCM
Reactivity

Human, Mouse

Summary

Nanog antibody recognizes Nanog protein, a 35 kDa transcription factor that maintains pluripotency and the self-renewal feature of embryonic stem cells. It is one of the Thomson factors (i.e., NANOG, OCT4, SOX2, and LIN28) that induce a stable intrinsic pluripotency network to generate induced pluripotent stem cells. In addition, NANOG expression is elevated in cancer stem cells and correlates with a worse prognosis for many tumor types.