Summary
Nanog antibody recognizes Nanog protein, a 35 kDa transcription factor that maintains pluripotency and the self-renewal feature of embryonic stem cells. It is one of the Thomson factors (i.e., NANOG, OCT4, SOX2, and LIN28) that induce a stable intrinsic pluripotency network to generate induced pluripotent stem cells. In addition, NANOG expression is elevated in cancer stem cells and correlates with a worse prognosis for many tumor types.
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
1:50-1:200 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , human ESC , mouse ESC , Tera-2
Predict Reactivity
Chimpanzee(>80% identity)
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_11169335
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.