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Nanog antibody [GT3312]

KO/KD
Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421
Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421
Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421
Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421
Anti-Nanog antibody [GT3312] used in Flow cytometry (FACS). GTX627421
Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421

Cat. No. GTX627421

Reference 25
3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT3312

Isotype

 IgG2b

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Nanog products
PRODUCT

Summary

Nanog antibody recognizes Nanog protein, a 35 kDa transcription factor that maintains pluripotency and the self-renewal feature of embryonic stem cells. It is one of the Thomson factors (i.e., NANOG, OCT4, SOX2, and LIN28) that induce a stable intrinsic pluripotency network to generate induced pluripotent stem cells. In addition, NANOG expression is elevated in cancer stem cells and correlates with a worse prognosis for many tumor types.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , human ESC , mouse ESC , Tera-2

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_11169335

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Nanog homeobox

Cellular Localization

Nucleus

Background

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

Database

Research Area

DATA IMAGES

Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421

GTX627421 WB Image

KO/KD

NANOG antibody [GT3312] validation by siRNA knock-down.
Upperpanel: NANOG antibody [GT3312] GTX627421
Lower panel: GAPDH antibody (GTX100118)
A. 30 μg Tera-2 whole cell lysate/extract
B. 30 μg whole cell lysate/extract of NANOG siRNA#1-transfected Tera-2 cells
C. 30 μg whole cell lysate/extract of NANOG siRNA#2-transfected Tera-2 cells
D. 30 μg whole cell lysate/extract of NANOG siRNA#3-transfected Tera-2 cells
10% SDS-PAGE
NANOG antibody [GT3312] (GTX627421) dilution: 1:1000
GAPDH antibody (GTX100118) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421

GTX627421 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
10% SDS PAGE
GTX627421 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421

GTX627421 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Nanog antibody [GT3312] (GTX627421) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421

GTX627421 WB Image

NANOG antibody [GT3312] detects NANOG protein by western blot analysis.
A. 30 μg human ESC whole cell lysate/extract
10% SDS-PAGE
NANOG antibody [GT3312] (GTX627421) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Nanog antibody [GT3312] used in Flow cytometry (FACS). GTX627421

GTX627421 FACS Image

NANOG antibody [GT3312] detects NANOG protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: NANOG antibody [GT3312] dilution: 1:50

Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421

GTX627421 WB Image

Sample (20 μg of whole cell lysate)
A: mouse ESC
10% SDS PAGE
GTX627421 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Peng Ye et al. Biomolecules 2022; 12 (5) Alanine-Glyoxylate Aminotransferase Sustains Cancer Stemness Properties through the Upregulation of SOX2 and OCT4 in Hepatocellular Carcinoma Cells.
Application : WB
Reactivity : Human
Hassan G et al. Sci Rep 2022; 12 (1) : 2711 The significance of ErbB2/3 in the conversion of induced pluripotent stem cells into cancer stem cells.
Application : WB
Reactivity : Human
Candice Merle et al. BMC Cancer 2021; 21 (1) : 241 Acquisition of cancer stem cell capacities after spontaneous cell fusion.
Application : WB
Reactivity : Human
Ciarpella F et al. iScience 2021; 24 (12) : 103438 Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity.
Reactivity : Mouse
Biagioni A et al. Front Oncol 2021; 11 : 663225 CRISPR/Cas9 uPAR Gene Knockout Results in Tumor Growth Inhibition, EGFR Downregulation and Induction of Stemness Markers in Melanoma and Colon Carcinoma Cell Lines.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

Nanog antibody [GT3312] Cat. No. GTX627421

Rating
( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Flow cytometry(FACS)
( Average 4 based on 1 users reviews)
Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cancers (Basel) in 2019. PMID: 31878324
Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Cancer in 2013. PMID: 24094005
Anti-Nanog antibody [GT3312] used in Flow cytometry (FACS). GTX627421
Date : Anonymous submitted on 11-Mar-2013
Score :
Application Tested : FACS
Sample Species : Hu
Sample : hES cells
Blocking : 2% serum, 4°C, 0.5Hr
Fixation : 70% ethanol
Primary Antibody : 1:50, 4°C, 0.5Hr
Figure Note : Black: Isotype control, Green: NANOG antibody
Submit a Review
Application Reference
Peng Ye et al. Biomolecules 2022; 12 (5) Alanine-Glyoxylate Aminotransferase Sustains Cancer Stemness Properties through the Upregulation of SOX2 and OCT4 in Hepatocellular Carcinoma Cells.
Application : WB
Reactivity : Human
Hassan G et al. Sci Rep 2022; 12 (1):2711 The significance of ErbB2/3 in the conversion of induced pluripotent stem cells into cancer stem cells.
Application : WB
Reactivity : Human
Candice Merle et al. BMC Cancer 2021; 21 (1):241 Acquisition of cancer stem cell capacities after spontaneous cell fusion.
Application : WB
Reactivity : Human
Ciarpella F et al. iScience 2021; 24 (12):103438 Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity.
Reactivity : Mouse
Biagioni A et al. Front Oncol 2021; 11:663225 CRISPR/Cas9 uPAR Gene Knockout Results in Tumor Growth Inhibition, EGFR Downregulation and Induction of Stemness Markers in Melanoma and Colon Carcinoma Cell Lines.
Application : WB
Reactivity : Human
Walker SJ et al. Heliyon 2021; 7 (4):e06617 A simplified approach for derivation of induced pluripotent stem cells from Epstein-Barr virus immortalized B-lymphoblastoid cell lines.
Application : ICC/IF
Reactivity : Human
Chen YL et al. Sci Rep 2021; 11 (1):644 Ephrin A4-ephrin receptor A10 signaling promotes cell migration and spheroid formation by upregulating NANOG expression in oral squamous cell carcinoma cells.
Application : WB
Reactivity : Human
Jao TM et al. Cancer Lett 2021; 499:290-300 PCDH10 exerts tumor-suppressor functions through modulation of EGFR/AKT axis in colorectal cancer.
Application : WB
Reactivity : Human
Ram?rez-Renter?a C et al. Case Rep Endocrinol 2020; 2020:4768281 From ACTH-Dependent to ACTH-Independent Cushing's Syndrome from a Malignant Mixed Corticomedullary Adrenal Tumor: Potential Role of Embryonic Stem Cells.
Lee KY et al. Cells 2020; 10 (1) Upregulation of CD109 Promotes the Epithelial-to-Mesenchymal Transition and Stemness Properties of Lung Adenocarcinomas via Activation of the Hippo-YAP Signaling.
Application : WB
Reactivity : Human
Blanas A et al. Cancers (Basel) 2020; 12 (9) FUT9-Driven Programming of Colon Cancer Cells towards a Stem Cell-Like State.
Application : FACS
Reactivity : Mouse
Andreucci E et al. J Mol Med (Berl) 2020; 98 (10):1431-1446 The acidic tumor microenvironment drives a stem-like phenotype in melanoma cells.
Application : FACS
Reactivity : Human
Chen YL et al. Cancers (Basel) 2019; 12 (1) ERK Activation Modulates Cancer Stemness and Motility of a Novel Mouse Oral Squamous Cell Carcinoma Cell Line.
Chen YH et al. Sci Rep 2019; 9 (1):9919 Mitochondrial Akt Signaling Modulated Reprogramming of Somatic Cells.
Application : ICC/IF
Reactivity : Human
Yang CE et al. Chem Biol Interact 2019; 302:28-35 the antipsychotic * suppresses yap signaling, stemness properties, and drug resistance in breast cancer cells.
Application : WB
Reactivity : Human
Lin YH et al. Endocr Relat Cancer 2018; 25 (12):967-979 Thyroid hormone negatively regulates tumorigenesis through suppression of BC200.
Application : WB
Reactivity : Human
Fan JR et al. Cell Death Dis 2018; 9 (5):502 Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model.
Application : FACS
Reactivity : Human
Zhang Y.X. et al. Transplantation Proceedings 2018; (Epub) Development of Individualized Induced Pluripotent Stem Cells from Fibroblasts of Keloid Lesions in Patients’.
Application : ICC/IF
Reactivity : Human
Chang KY et al. Biochem Biophys Res Commun 2017; 493 (1):14-19 Stress stimuli induce cancer-stemness gene expression via Sp1 activation leading to therapeutic resistance in glioblastoma.
Application : WB
Reactivity : Human
Ko CY et al. Mol Neurobiol 2017; (Epub) MDM2 Degrades Deacetylated Nucleolin Through Ubiquitination to Promote Glioma Stem-Like Cell Enrichment for Chemotherapeutic Resistance.
Application : WB
Reactivity : Human
Gupta N et al. Proc Natl Acad Sci U S A 2017; 114 (5):1033-1038 Stress granule-associated protein G3BP2 regulates breast tumor initiation.
Application : WB
Reactivity : Human
Huang TT et al. Oncotarget 2015; 6 (20):18012-26 The reciprocal regulation loop of Notch2 pathway and miR-23b in controlling gastric carcinogenesis.
Application : WB
Reactivity : Human
Wang AM et al. Oncotarget 2014; 5 (13):5002-16 Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis.
Application : WB
Reactivity : Human
Liu KC et al. Oncotarget 2013; 4 (12):2366-82 Ovarian cancer stem-like cells show induced translineage-differentiation capacity and are suppressed by alkaline phosphatase inhibitor.
Application : FACS
Reactivity : Human
Huang AF et al. Mol Cancer 2013; 12 (1):115 CD164 regulates the tumorigenesis of ovarian surface epithelial cells through the SDF-1α/CXCR4 axis.
Application : WB
Reactivity : Human
SDS
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