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Nanog antibody [N3C3]

Anti-Nanog antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100863
Anti-Nanog antibody [N3C3] used in ELISA (ELISA). GTX100863
Anti-Nanog antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100863
Anti-Nanog antibody [N3C3] used in Immunoprecipitation (IP). GTX100863
Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863
Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863

Cat No. GTX100863

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, IHC, sELISA

Reactivity

Human
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr Assay dependent
IP 1:100-1:500
ELISA 1:1000-1:10000
IHC Assay dependent
sELISA Assay dependent

Note :

sELISA
Capture: GTX100863 , Detection: GTX89079

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

HeLa nucleus , NIH-3T3 , mouse ESC

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Nanog homeobox

Cellular Localization

Nucleus

Background

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

Database

Research Area

DATA IMAGES

Anti-Nanog antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100863

GTX100863 ICC/IF Image

NANOG antibody [N3C3] detects NANOG protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min.
Green: NANOG protein stained by NANOG antibody [N3C3] (GTX100863) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 6 μm.

Anti-Nanog antibody [N3C3] used in ELISA (ELISA). GTX100863

GTX100863 ELISA Image

ELISA detection of NANOG using GTX100863 for capture at a concentration of 5 μg/mL and GTX89079 for detection at a concentration of 5 μg/mL.

Anti-Nanog antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100863

GTX100863 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using NANOG(GTX100863) antibody at 1:400 dilution.

Anti-Nanog antibody [N3C3] used in Immunoprecipitation (IP). GTX100863

GTX100863 IP Image

Immunoprecipitation of NANOG protein from NT2D1 whole cell extracts using 5 μg of NANOG antibody [N3C3] (GTX100863).
Western blot analysis was performed using NANOG antibody [N3C3] (GTX100863).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863

GTX100863 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa nucleus
12% SDS PAGE
GTX100863 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863

GTX100863 WB Image

Thiostrepton suppresses tumor sphere- and colony-formation potentials of HCT-15 and HT-29 cells as well as the expression of factors involved in stemness maintenance. (a) HCT-15 and HT-29 cells were cultured in defined media supplemented continuously with DMSO (Veh), oxaliplatin (Oxa, 25 μM), or thiostrepton (Thio, 5 μM). Seven days later, spheres stained by MTT were photographed ( × 40) and counted using software. *P<0.05 when compared with the DMSO-treated cells by Student's t-test. (b) Clonogenic assays were performed using cells exposed previously to DMSO (Ctrl), oxaliplatin, or thiostrepton for 6 h as described in ‘Materials and Methods'. Ten days after seeding, colonies were stained by crystal violet and their numbers were counted using Colony 1.1 software. *P<0.05 when compared with the DMSO-treated cells by Student's t-test. Total lysates prepared from (c) Snail OE and (d) r29 cells after being treated with the indicated doses of thiostrepton for 48 h were subjected to western blot analyses using antibodies against CD44, ALDH1, Oct4, Nanog, Bmi1, and Twist as probes. Tubulin signal served as a loading control

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169