GeneTex
United States (US)

Nanog antibody [N3C3]

GTX100863_40625_20150513_IFA_19011506_375.jpg
NANOG_ELISA-PAIR_19011506_421.jpg
GTX100863_40359_IFA_hESC_19011506_758.jpg
GTX100863_40436_IP_19011506_604.jpg
GTX100863_40436_WB_M_19011506_480.jpg
GTX100863_40436_WB_M_ESC_19011506_129.jpg
GTX100863_40625_WB_19011506_315.jpg

Cat No. GTX100863

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, IHC
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr Assay dependent
IP 1:100-1:500
ELISA 1:1000-1:10000
IHC Assay dependent
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

HeLa nucleus , NIH-3T3 , mouse ESC

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nanog Homeobox,Nanog

Cellular Localization

Nucleus

Background

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

Database

Research Area

DATA IMAGES
GTX100863_40625_20150513_IFA_19011506_375.jpg

GTX100863 ICC/IF Image

NANOG antibody [N3C3] detects NANOG protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min.
Green: NANOG protein stained by NANOG antibody [N3C3] (GTX100863) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 6 μm.

NANOG_ELISA-PAIR_19011506_421.jpg

GTX100863 ELISA Image

ELISA detection of NANOG using GTX100863 for capture at a concentration of 5 µg/mL and GTX89079 for detection at a concentration of 5 µg/mL.

GTX100863_40359_IFA_hESC_19011506_758.jpg

GTX100863 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using NANOG(GTX100863) antibody at 1:400 dilution.

GTX100863_40436_IP_19011506_604.jpg

GTX100863 IP Image

Immunoprecipitation of NANOG protein from NT2D1 whole cell extracts using 5 μg of NANOG antibody [N3C3] (GTX100863).
Western blot analysis was performed using NANOG antibody [N3C3] (GTX100863).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX100863_40436_WB_M_19011506_480.jpg

GTX100863 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX100863 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100863_40436_WB_M_ESC_19011506_129.jpg

GTX100863 WB Image

Sample (20 µg of whole cell lysate)
A: mouse ESC
12% SDS PAGE
GTX100863 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100863_40625_WB_19011506_315.jpg

GTX100863 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa nucleus
12% SDS PAGE
GTX100863 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169