Nanog antibody [N3C3]

Nanog antibody [N3C3] detects Nanog protein by immunofluorescent analysis.
Sample: PANC-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nanog stained by Nanog antibody [N3C3] (GTX100863) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Nanog antibody [N3C3] detects Nanog protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 2% paraformaldehyde at RT for 15 min.
Green: Nanog stained by Nanog antibody [N3C3] (GTX100863) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

ELISA detection of NANOG using GTX100863 for capture at a concentration of 5 μg/mL and GTX89079 for detection at a concentration of 5 μg/mL.

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using NANOG(GTX100863) antibody at 1:400 dilution.

Immunoprecipitation of NANOG protein from NT2D1 whole cell extracts using 5 μg of NANOG antibody [N3C3] (GTX100863).
Western blot analysis was performed using NANOG antibody [N3C3] (GTX100863) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Sample (30 μg of whole cell lysate)
A: HeLa nucleus
12% SDS PAGE
GTX100863 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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The data was published in the journal Cell Death Dis in 2015. PMID: 26136074

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The data was published in the journal Elife in 2017.PMID: 28537559

The data was published in the journal Elife in 2017.PMID: 28537559