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Nanog antibody [N3C3]

Orthogonal
Anti-Nanog antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100863
Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863
Anti-Nanog antibody [N3C3] used in Immunoprecipitation (IP). GTX100863
Anti-Nanog antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100863
Anti-Nanog antibody [N3C3] used in ELISA (ELISA). GTX100863

Cat. No. GTX100863

Reference 33
7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, IHC, Sandwich ELISA

Reactivity

 Human, Mouse, Ceratotherium simum
Package
100 μl ($399),
25 μl ($169)
See all Nanog products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr Assay dependent
IP 1:100-1:500
ELISA 1:1000-1:10000
IHC Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX100863 , Detection: GTX89079
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

HeLa nucleus

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10615506

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Nanog homeobox

Cellular Localization

Nucleus

Background

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

Database

Research Area

DATA IMAGES

Anti-Nanog antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100863

GTX100863 ICC/IF Image

NANOG antibody [N3C3] detects NANOG protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min.
Green: NANOG protein stained by NANOG antibody [N3C3] (GTX100863) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 6 μm.

Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863

GTX100863 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa nucleus
12% SDS PAGE
GTX100863 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Nanog antibody [N3C3] used in Immunoprecipitation (IP). GTX100863

GTX100863 IP Image

Immunoprecipitation of NANOG protein from NT2D1 whole cell extracts using 5 μg of NANOG antibody [N3C3] (GTX100863).
Western blot analysis was performed using NANOG antibody [N3C3] (GTX100863) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Nanog antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100863

GTX100863 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using NANOG(GTX100863) antibody at 1:400 dilution.

Anti-Nanog antibody [N3C3] used in ELISA (ELISA). GTX100863

GTX100863 ELISA Image

ELISA detection of NANOG using GTX100863 for capture at a concentration of 5 μg/mL and GTX89079 for detection at a concentration of 5 μg/mL.

REFERENCE

Application Reference

Mohankumar K et al. Mol Carcinog 2022; 61 (1) : 73-84 Nuclear receptor 4A1 (NR4A1) antagonists target paraspeckle component 1 (PSPC1) in cancer cells.
Application : WB
Reactivity : Human
Huang H et al. Sci Adv 2022; 8 (28) : eabo5668 Cis-regulatory chromatin loops analysis identifies GRHL3 as a master regulator of surface epithelium commitment.
Masola V et al. Front Oncol 2022; 12 : 918419 Heparanase regulates EMT and cancer stem cell properties in prostate tumors.
Application : WB
Reactivity : Human
Fabio Papes et al. Nat Commun 2022; 13 (1) : 2387 Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content.
Application : ICC/IF
Seung-Hun Kim et al. Cancer Drug Resist 2021; 4 (4) : 866-880 The aqueous extract of brucea javanica reduces tumorigenicity of human lung cancer tumorspheres.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

Nanog antibody [N3C3] Cat. No. GTX100863

Rating
( Average 5 based on 7 users reviews)
Application
Western Blot(WB)
( Average 5 based on 5 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 2 users reviews)
Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Biomed Sci in 2016.PMID: 27863490
Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Elife in 2017.PMID: 28537559
Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Elife in 2017.PMID: 28537559
Anti-Nanog antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100863
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2020.PMID: 32256979
Anti-Nanog antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100863
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Ceratotherium simum
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2018. PMID: 29973581
Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2015. PMID: 26136074
Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2015. PMID: 26136074
Show More +
Submit a Review
Application Reference
Mohankumar K et al. Mol Carcinog 2022; 61 (1):73-84 Nuclear receptor 4A1 (NR4A1) antagonists target paraspeckle component 1 (PSPC1) in cancer cells.
Application : WB
Reactivity : Human
Huang H et al. Sci Adv 2022; 8 (28):eabo5668 Cis-regulatory chromatin loops analysis identifies GRHL3 as a master regulator of surface epithelium commitment.
Masola V et al. Front Oncol 2022; 12:918419 Heparanase regulates EMT and cancer stem cell properties in prostate tumors.
Application : WB
Reactivity : Human
Fabio Papes et al. Nat Commun 2022; 13 (1):2387 Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content.
Application : ICC/IF
Seung-Hun Kim et al. Cancer Drug Resist 2021; 4 (4):866-880 The aqueous extract of brucea javanica reduces tumorigenicity of human lung cancer tumorspheres.
Application : WB
Reactivity : Human
Zhang W et al. Stem Cell Res 2021; 57:102611 Generation of a DKK2 homozygous knockout human embryonic stem cell line using the CRISPR/Cas9 system.
Application : ICC/IF
Reactivity : Human
Mashiko T et al. Cells 2021; 10 (3) Selective Proliferation of Highly Functional Adipose-Derived Stem Cells in Microgravity Culture with Stirred Microspheres.
Application : ICC/IF
Reactivity : Human
Wang SM et al. Cell Death Discov 2021; 7 (1):8 CCAAT/Enhancer-binding protein delta mediates glioma stem-like cell enrichment and ATP-binding cassette transporter ABCA1 activation for temozolomide resistance in glioblastoma.
Application : WB
Reactivity : Human
Carelli S et al. Nanotheranostics 2021; 5 (1):8-26 Neural precursors cells expanded in a 3D micro-engineered niche present enhanced therapeutic efficacy in vivo.
Application : WB
Reactivity : Mouse
Assoun EN et al. Oncotarget 2020; 11 (12):1075-1084 Characterization of iPS87, a prostate cancer stem cell-like cell line.
Tsai LH et al. Sci Rep 2020; 10 (1):17817 Targeting interleukin-17 receptor B enhances gemcitabine sensitivity through downregulation of mucins in pancreatic cancer.
Application : WB
Reactivity : Human
Peng F et al. Cell Res 2020; (Epub) Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.
Application : WB, IHC-P
Reactivity : Human
Liu Z et al. Am J Transl Res 2020; 12 (6):2538-2553 LINC01619 promotes non-small cell lung cancer development via regulating PAX6 by suppressing microRNA-129-5p.
Application : WB
Reactivity : Human
Raineri A et al. Int J Mol Sci 2019; 20 (23) Onconase Restores Cytotoxicity in Dabrafenib-Resistant A375 Human Melanoma Cells and Affects Cell Migration, Invasion and Colony Formation Capability.
Huang JY et al. Cancers (Basel) 2019; 12 (1) Visfatin Mediates Malignant Behaviors through Adipose-Derived Stem Cells Intermediary in Breast Cancer.
Application : WB
Reactivity : Human
Liu LP et al. Cell Rep 2019; 27 (2):455-466.e5 Therapeutic Potential of Patient iPSC-Derived iMelanocytes in Autologous Transplantation.
Application : ICC/IF
Hsu CF et al. EBioMedicine 2019; 41:597-609 IGF-axis confers transformation and regeneration of fallopian tube fimbria epithelium upon ovulation.
Application : IHC-P
Reactivity : Human
Xu J et al. Stem Cell Res 2019; 35:101407 Generation of a human Charcot-Marie-Tooth disease type 1B (CMT1B) iPSC line, ZJUCHi001-A, with a mutation of c.292C>T in MPZ.
Application : ICC/IF
Reactivity : Human
Hildebrandt TB et al. Nat Commun 2018; 9 (1):2589 Embryos and embryonic stem cells from the white rhinoceros.
Application : ICC/IF
Di Stefano AB et al. J Cell Physiol 2018; (Epub) Spheroids from adipose-derived stem cells exhibit an miRNA profile of highly undifferentiated cells.
Application : IHC-P
Reactivity : Human
Shen Z et al. Elife 2017; 6 Enforcement of developmental lineage specificity by transcription factor Oct1.
Application : WB
Ni YL et al. Apoptosis 2017; (Epub) A potent indolylquinoline alleviates growth of human lung cancer cell tumorspheres.
Application : WB
Reactivity : Human
Hsu CC et al. J Biomed Sci 2016; 23 (1):81 Suberoylanilide hydroxamic acid represses glioma stem-like cells.
Emhemmed F et al. Cell Biol Toxicol 2016; (Epub) Pro-differentiating effects of a synthetic flavagline on human teratocarcinomal cancer stem-like cells.
Application : ICC/IF
Reactivity : Human
Mineda K et al. Stem Cells Transl Med 2015; 4 (12):1511-22 Therapeutic Potential of Human Adipose-Derived Stem/Stromal Cell Microspheroids Prepared by Three-Dimensional Culture in Non-Cross-Linked Hyaluronic Acid Gel.
Application : IHC-Fr
Reactivity : Human
Ju SY et al. Cell Death Dis 2015; 6 (Epub):e1801 identification of * as a novel therapeutic agent that targets human colon cancer stem cells.
Application : WB
Reactivity : Human
Marinho PA et al. Sci Rep 2015; 5:9834 Systematic optimization of human pluripotent stem cells media using Design of Experiments.
Application : ICC/IF
Reactivity : Human
Liu JT et al. Toxicol Appl Pharmacol 2014; 281 (3):243-53 Arsenic inhibits hedgehog signaling during P19 cell differentiation.
Jui Tung Liu et al. Toxicology and Applied Pharmacology 2014; Arsenic inhibits hedgehog signaling during P19 cell differentiation
Application : IHC
Emhemmed F et al. Biochem Pharmacol 2014; Selective anticancer effects of a synthetic flavagline on human Oct4-expressing cancer stem-like cells via a p38 MAPK-dependent caspase-3-dependent pathway.
Application : WB
Reactivity : Human
Singh AM et al. Stem Cell Reports 2013; 1 (6):532-44 Cell-cycle control of developmentally regulated transcription factors accounts for heterogeneity in human pluripotent cells.
Menendez L et al. Nat Protoc 2013; 8 (1):203-12 Directed differentiation of human pluripotent cells to neural crest stem cells.
Application : ICC/IF
Reactivity : Human
Hong GM et al. Toxicol Sci 2012; 129 (1):146-56 Arsenic exposure inhibits myogenesis and neurogenesis in P19 stem cells through repression of the β-catenin signaling pathway.
Application : ICC/IF
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169