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Nanog antibody [N3C3]

Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863
Anti-Nanog antibody [N3C3] used in Immunoprecipitation (IP). GTX100863
Anti-Nanog antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100863
Anti-Nanog antibody [N3C3] used in ELISA (ELISA). GTX100863
Anti-Nanog antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100863
Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863
Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863
Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863
Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863
Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863
Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863
Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863
Anti-Nanog antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100863
Anti-Nanog antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100863

Cat. No. GTX100863

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, IHC, Sandwich ELISA

Reactivity

Human, Mouse, Ceratotherium simum
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr Assay dependent
IP 1:100-1:500
ELISA 1:1000-1:10000
IHC Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX100863 , Detection: GTX89079

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

HeLa nucleus

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10615506

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Nanog homeobox

Cellular Localization

Nucleus

Background

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

Database

Research Area

DATA IMAGES

Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863

GTX100863 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa nucleus
12% SDS PAGE
GTX100863 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Nanog antibody [N3C3] used in Immunoprecipitation (IP). GTX100863

GTX100863 IP Image

Immunoprecipitation of NANOG protein from NT2D1 whole cell extracts using 5 μg of NANOG antibody [N3C3] (GTX100863).
Western blot analysis was performed using NANOG antibody [N3C3] (GTX100863) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Nanog antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100863

GTX100863 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using NANOG(GTX100863) antibody at 1:400 dilution.

Anti-Nanog antibody [N3C3] used in ELISA (ELISA). GTX100863

GTX100863 ELISA Image

ELISA detection of NANOG using GTX100863 for capture at a concentration of 5 μg/mL and GTX89079 for detection at a concentration of 5 μg/mL.

Anti-Nanog antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100863

GTX100863 ICC/IF Image

Nanog antibody [N3C3] detects Nanog protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 2% paraformaldehyde at RT for 15 min.
Green: Nanog stained by Nanog antibody [N3C3] (GTX100863) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863

GTX100863 WB Image

The data was published in the journal J Biomed Sci in 2016.PMID: 27863490

Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863

GTX100863 WB Image

The data was published in the 2021 in Cancer Drug Resist. PMID: 35582384

Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863

GTX100863 WB Image

The data was published in the 2019 in Cancers (Basel). PMID: 31861872

Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863

GTX100863 WB Image

The data was published in the journal Cell Death Dis in 2015. PMID: 26136074

Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863

GTX100863 WB Image

The data was published in the journal Cell Death Dis in 2015. PMID: 26136074

Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863

GTX100863 WB Image

The data was published in the journal Elife in 2017.PMID: 28537559

Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863

GTX100863 WB Image

The data was published in the journal Elife in 2017.PMID: 28537559

Anti-Nanog antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100863

GTX100863 ICC/IF Image

The data was published in the journal Oncotarget in 2020.PMID: 32256979

Anti-Nanog antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100863

GTX100863 ICC/IF Image

The data was published in the journal Nat Commun in 2018. PMID: 29973581

REFERENCE

REVIEW

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Application Reference
Tsai LH et al. Sci Rep 2020; 10 (1):17817
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169